Difference between revisions of "DNA Melting Report Requirements for Part 1"
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+ | ==Report requirements== | ||
#Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. | #Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. | ||
#Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out. | #Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out. | ||
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#Obtain a DNA melting curve by plotting fluorescence versus temperature. | #Obtain a DNA melting curve by plotting fluorescence versus temperature. | ||
##Repeat two more times. | ##Repeat two more times. | ||
− | ##On | + | ##On one graph, plot your three melting curves. |
#Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit. | #Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit. | ||
##What values do you obtain for ΔH and ΔS? | ##What values do you obtain for ΔH and ΔS? |
Revision as of 23:41, 2 December 2011
Report requirements
- Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance.
- Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out.
- What is the signal to noise ratio (SNR) in units of dB for your system?
- Ensure that your system has an SNR of at least 12 dB.
- Obtain a DNA melting curve by plotting fluorescence versus temperature.
- Repeat two more times.
- On one graph, plot your three melting curves.
- Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit.
- What values do you obtain for ΔH and ΔS?
Lab manual sections
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2