Difference between revisions of "DNA Melting Report Requirements for Part 1"
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| − | Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. Using the | + | #Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance. |
| − | same amplifier gain, estimate the change in signal between DNA sample in and sample out. What is the signal | + | #Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out. |
| − | to noise ratio (SNR) in units of dB for your system? | + | ##What is the signal to noise ratio (SNR) in units of dB for your system? |
| − | Ensure that your system has an SNR of at least 12 dB. | + | ##Ensure that your system has an SNR of at least 12 dB. |
| − | Obtain a DNA melting curve by plotting fluorescence versus temperature. Repeat two more times. On the | + | #Obtain a DNA melting curve by plotting fluorescence versus temperature. |
| − | same graph, plot your three melting curves. | + | ##Repeat two more times. |
| − | Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares | + | ##On the same graph, plot your three melting curves. |
| − | fit. What values do you obtain for ΔH and ΔS? | + | #Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit. |
| + | ##What values do you obtain for ΔH and ΔS? | ||
==Lab manual sections== | ==Lab manual sections== | ||
Revision as of 23:40, 2 December 2011
- Plot fluorescence versus time for one minute and estimate the noise level by calculating the variance.
- Using the same amplifier gain, estimate the change in signal between DNA sample in and sample out.
- What is the signal to noise ratio (SNR) in units of dB for your system?
- Ensure that your system has an SNR of at least 12 dB.
- Obtain a DNA melting curve by plotting fluorescence versus temperature.
- Repeat two more times.
- On the same graph, plot your three melting curves.
- Pick your favorite of the three melting curves and use your code from the simulation to perform a least squares fit.
- What values do you obtain for ΔH and ΔS?
Lab manual sections
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
