Difference between revisions of "DNA Melting Report Requirements for Part 1"
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# Report the estimated melting temperature and the best-fit values of ''ΔH°'', ''ΔS°''. | # Report the estimated melting temperature and the best-fit values of ''ΔH°'', ''ΔS°''. | ||
# Report your signal to noise measurement. | # Report your signal to noise measurement. | ||
+ | # Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab. | ||
==Lab manual sections== | ==Lab manual sections== |
Revision as of 13:34, 10 May 2015
Report requirements
- One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example:
CrickFranklinWatson.pdf
. - The file must be less than 20 MB.
- Include code at the end of the document in an appendix.
Part 1 report outline
- Document your circuit design, optical design, and any ways that your instrument differs from the system described in the lab manual.
- Plot at least one melting curve.
- The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
- On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
- Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters.
- Include a legend.
- Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
- Report your signal to noise measurement.
- Explain the statistical method you will use to identify your group's unknown sample in part 2 of this lab.
Lab manual sections
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
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