Difference between revisions of "DNA Melting Report Requirements for Part 1"

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==Report requirements==
 
==Report requirements==
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# Report submittal format:
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* One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example: <code>CrickFranklinWatson.pdf</code>.
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#;Size of your lab report file submitted MUST be less than 20 MB.
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* The file must be less than 20 MB.
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#;Post only 1 single .pdf document, with code included in the pdf at the end, not separately.
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* Include code at the end of the document in an appendix.
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#;Include last name of each group member in the '''filename''' of the pdf you post. We would rather spend time carefully grading your report than renaming your files because every other group named theirs "DNA Report Part 1" or the like.
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#Report your signal to noise measurement.
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==Part 1 report outline==
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#Plot your favorite melting curves.
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# Document your circuit design, optical design, and any changes you made to the system described in the lab manual.
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#For at least one of your data curves (or an average of your best curves), use your code from the simulation to perform a least-squares fit and comment on the results.  
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# Plot at least one melting curve.  
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#*What values do you obtain for ΔH and ΔS? Do you trust these values?
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#* The plot should have temperature in &deg;C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
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#*Estimate the melting temperature based on the plot.
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#* On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
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#* Also on the same set of axes, plot the output of the <tt>DnaFraction</tt> function evaluated with best-fit parameters.
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#* Include a legend.
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# Report the estimated melting temperature and the best-fit values of ''&Delta;H&deg;'', ''&Delta;S&deg;''.
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# Report your signal to noise measurement.
  
 
==Lab manual sections==
 
==Lab manual sections==

Revision as of 11:00, 23 April 2015

20.309: Biological Instrumentation and Measurement

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Report requirements

  • One member of your group should submit a single PDF file to Stellar in advance of the deadline. The filename should consist of the last names of all group members, CamelCased, in alphabetical order, with a .pdf extension. Example: CrickFranklinWatson.pdf.
  • The file must be less than 20 MB.
  • Include code at the end of the document in an appendix.

Part 1 report outline

  1. Document your circuit design, optical design, and any changes you made to the system described in the lab manual.
  2. Plot at least one melting curve.
    • The plot should have temperature in °C on the horizontal axis and fraction of double stranded DNA on the vertical axis.
    • On the same set of axes, include a simulated curve generated by DINAMelt, OligoCalc, or another software simulator.
    • Also on the same set of axes, plot the output of the DnaFraction function evaluated with best-fit parameters.
    • Include a legend.
  3. Report the estimated melting temperature and the best-fit values of ΔH°, ΔS°.
  4. Report your signal to noise measurement.

Lab manual sections

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