Difference between revisions of "Assignment 9 Overview: Analyzing yeast images"
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In Assignment 8 you upgraded your microscope to make two-color images, and fabricated a microfluidic device that we used to switch the flow through the device from two fluid reservoirs. In Assignment 10, we'll use this new setup to replicate part of the experiment described in the following paper: | In Assignment 8 you upgraded your microscope to make two-color images, and fabricated a microfluidic device that we used to switch the flow through the device from two fluid reservoirs. In Assignment 10, we'll use this new setup to replicate part of the experiment described in the following paper: | ||
− | *[http://science.sciencemag.org/content/319/5862/482 | + | *[http://science.sciencemag.org/content/319/5862/482 J. T. Mettetal, D. Muzzey, C. Gomez-Uribe, and A. van Oudenaarden, "The Frequency Dependence of Osmo-Adaptation in Saccharomyces cerevisiae," Science, vol. 319, no. 5862, pp. 482–484, 2008] |
In preparation for this experiment, we're going to write the analysis code needed to extract the Hog1 protein's response from images of yeast cells. | In preparation for this experiment, we're going to write the analysis code needed to extract the Hog1 protein's response from images of yeast cells. |
Revision as of 20:56, 13 November 2018
Overview
In Assignment 8 you upgraded your microscope to make two-color images, and fabricated a microfluidic device that we used to switch the flow through the device from two fluid reservoirs. In Assignment 10, we'll use this new setup to replicate part of the experiment described in the following paper:
In preparation for this experiment, we're going to write the analysis code needed to extract the Hog1 protein's response from images of yeast cells.
To get started, read Mettetal's paper.
Next, head over to Part 1 of this assignment, where you'll answer some questions about the paper and write your analysis code:
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment9.pdf.