Difference between revisions of "Assignment 5 Overview"
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For a slide of fixed beads: | For a slide of fixed beads: | ||
# Plot the MSD versus time interval of | # Plot the MSD versus time interval of | ||
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#* the sum trajectories, and | #* the sum trajectories, and | ||
#* the difference trajectories. | #* the difference trajectories. | ||
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#* What differences do you see between the untreated and Cyto D treated MSD curves? | #* What differences do you see between the untreated and Cyto D treated MSD curves? | ||
#* Please suggest an interpretation of the behavior of your cells based on your data. | #* Please suggest an interpretation of the behavior of your cells based on your data. | ||
− | #* Include a discussion of your error sources. | + | #* #* Include a thorough discussion of error sources and your approaches to minimize them. As in Assignment 4, list out the error sources in a table, including a category for the error source, type of error (random, systematic, fundamental, technical, etc.), the magnitude of the error, and a description and way to minimize each one. Your MSD measurements of the still beads (from Part 1 of this assignment) will be useful for estimating the magnitude of several significant error sources. |
+ | |||
+ | Include any MATLAB code you've written as an appendix to your assignment. | ||
}} | }} | ||
Revision as of 18:23, 11 March 2019
Assignment details
This assignment has 2 parts:
- Part 1: Implementing difference tracking and measuring the stability of your microscope;
- Part 2: Live cell particle tracking of endocytosed beads.
Submit your work in on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment5.pdf.
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
- Overview
- Part 1: MSD difference tracking and microscope stability
- Part 2: Live cell particle tracking of endocytosed beads
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