Assignment 3 Overview

Assignment details

This assignment has 2 parts:

1. Part 1: Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-field correction code you wrote for Assignment 2 to address non-uniform illumination;
2. Part 2: Explore interesting calculations and considerations to guide your experimental design with fluorescence.

Turn in all of your work on Stellar in a single PDF file named <lastname><firstname>Assignment3.pdf.

Background reading

• Flat-field correction of non-uniform illumination
• Geometrical optics and ray tracing
• Physical optics and resolution
• Optical aberrations
• Aperture and field stops
• Optical detectors, noise, and the limit of detection
• Manta G032 camera measurements
• Understanding log plots