Difference between revisions of "Assignment 3 Overview"

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===Background reading===
 
===Background reading===
 
* [[Flat-field correction|Flat-field correction of non-uniform illumination]]
 
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* [[Geometrical optics and ray tracing]]
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* [[Physical optics and resolution]]
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* [[Optical aberrations]]
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* [[Aperture and field stops]]
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* [[Optical detectors, noise, and the limit of detection]]
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* [[Manta G032 camera measurements]]
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* [[Understanding log plots]]
 
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Revision as of 20:56, 14 August 2017

20.309: Biological Instrumentation and Measurement

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Assignment details

This assignment has 2 parts:

  1. Part 1: Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-field correction code you wrote for Assignment 2 to address non-uniform illumination;
  2. Part 2: Explore interesting calculations and considerations to guide your experimental design with fluorescence;

Turn in all of your work on Stellar in a single PDF file named <lastname><firstname>Assignment3.pdf.

Background reading