Difference between revisions of "Assignment 2: fluorescence microscopy"
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− | # | + | # Draw a block diagram of the LED epi-illuminaton path. Indicate the focal lengths of all lenses, the correct lens orientation, and all important distances between components. |
− | + | # Lenses L3 and L4 make an image of the LED. Assuming the initial size of the LED is 1.3 mm, what is the size of the LED image made by lens L3? | |
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# For each bead sample, include the original, reference, and flat-field corrected images in your lab report. In the caption note the exposure and gain settings used for each image. | # For each bead sample, include the original, reference, and flat-field corrected images in your lab report. In the caption note the exposure and gain settings used for each image. | ||
# For one set of images (either the 0.84 or 3.6 μm beads and their corresponding dark and reference images), include the MATLAB code you used to calculate the flat-field correction. | # For one set of images (either the 0.84 or 3.6 μm beads and their corresponding dark and reference images), include the MATLAB code you used to calculate the flat-field correction. |
Revision as of 13:50, 11 September 2018
This assignment has 3 parts.
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment2.pdf.
- Overview
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
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