Difference between revisions of "Assignment 2: fluorescence microscopy"
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This assignment has 3 parts. | This assignment has 3 parts. | ||
− | + | * [[Assignment 2 Part 1: Noise in images|Part 1: Noise in images]] | |
− | + | * [[Assignment 2 Part 2: Fluorescence microscopy|Part 2: Fluorescence microscopy]] | |
+ | * [[Assignment 2 Part 3: Build an epi-illuminator for your microscope|Part 3: Build an epi-illuminator for your microscope]] | ||
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment2.pdf. | Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment2.pdf. | ||
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# Repeat the simulation for a detector that has only a 25% chance of detecting a photon and a light source that is 4 time brighter. | # Repeat the simulation for a detector that has only a 25% chance of detecting a photon and a light source that is 4 time brighter. | ||
# Turn in the code you used to generate the plots | # Turn in the code you used to generate the plots | ||
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# On one set of axes, plot the average variance vs. mean for exposure times of 10<sup>-4</sup>, 10<sup>-3</sup>, 10<sup>-2</sup>, 10<sup>-1</sup>, 10<sup>0</sup> seconds. | # On one set of axes, plot the average variance vs. mean for exposure times of 10<sup>-4</sup>, 10<sup>-3</sup>, 10<sup>-2</sup>, 10<sup>-1</sup>, 10<sup>0</sup> seconds. | ||
# In what situations is dark current noise a significant problem? | # In what situations is dark current noise a significant problem? | ||
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# For one set of images (either the 0.84 or 3.6 μm beads and their corresponding dark and reference images), include the MATLAB code you used to calculate the flat-field correction. | # For one set of images (either the 0.84 or 3.6 μm beads and their corresponding dark and reference images), include the MATLAB code you used to calculate the flat-field correction. | ||
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+ | {{Template:Assignment 2 navigation}} | ||
{{Template:20.309 bottom}} | {{Template:20.309 bottom}} |
Revision as of 17:51, 19 September 2017
This assignment has 3 parts.
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment2.pdf.
- Overview
- Part 1: Noise in images
- Part 2: Fluorescence microscopy
- Part 3: Build an epi-illuminator for your microscope
Back to 20.309 Main Page