20.109: Spring 2007 schedule

20.109: Laboratory Fundamentals of Biological Engineering

Home People Schedule Spring 2007 Lab Basics OWW Basics
Genome Engineering Biophysical Signal Measurement Expression Engineering Biomaterial Engineering

MODULE	DAY	DATE	LECTURER	TA	LAB
		Tues Feb 6	NK		Orientation

1	1	Thurs Feb 8	NK	YSN	DNA engineering by PCR
1	2	Tues Feb 13	NK	YSN	Clean and cut DNA
1	3	Thurs Feb 15	NK	YSN	Agarose gel electrophoresis
		President's Day Feb 19			No lab Feb 20 (Mon schedule) or Feb 21
1	4	Thurs Feb 22	NK	YSN	DNA ligation and bacterial transformation
1	5	Tues Feb 27	NK	YSN	Examine candidate clones
1	6	Thurs Mar 1	NK	YSN	Restriction map+ Article Discussion/Tissue Culture

2	1	Tues Mar 6	AJ	AT	Lipofection
2	2	Thurs Mar 8	AJ	AT	FACS analysis
2	3	Tues Mar 13	AJ	AT	Tools for protein engineering
2	4	Thurs Mar 15	AJ	AT	Assessing beta-galactosidase
2	5	Tues Mar 20	AJ	AT	Purifying beta-galactosidase
2	6	Thurs Mar 22	AJ	AT	Student presentations
		Spring Break Mar 26-30			No lab all week

3	1	Tues Apr 3	DE	SC	Tools for systems engineering
3	2	Thurs Apr 5	DE	SC	Basic bacterial photography: black and white
3	3	Tues Apr 10	DE	SC	Advanced bacterial photography: 2 color
3	4	Thurs Apr 12	DE	SC	Device characterization
		Patriot's Day April 16-17			No lab Apr 17 or Apr 18
3	5	Thurs Apr 19	DE	SC	Measuring DNA, RNA, protein
3	6	Tues Apr 24	DE	SC	RT-PCR data analysis

4	1	Thurs Apr 26	AB	CV	Screening library
4	2	Tues May 1	AB	CV	Optimizing panning
4	3	Thurs May 3	AB	CV	Rescreening gold binders
4	4	Tues May 8	AB	CV	PCR of gold binding candidates
4	5	Thurs May 10	AB	CV	Student presentations
4	6	Tues May 15	AB	CV	Analyze sequence data
		Thurs May 18			Lecture only, no lab

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