20.109: Spring 2007 schedule

20.109: Laboratory Fundamentals of Biological Engineering

Home People Schedule Spring 2007 Lab Basics OWW Basics
Genome Engineering Biophysical Signal Measurement Expression Engineering Biomaterial Engineering

MODULE	DAY	DATE	LECTURER	TA	LAB
		Tues Feb 6	NK		Orientation

1	1	Thurs Feb 8			DNA engineering by PCR
1	2	Tues Feb 13			Clean and cut DNA
1	3	Thurs Feb 15			Agarose gel electrophoresis
		President's Day Feb 19			No lab Feb 20 (Mon schedule) or Feb 21
1	4	Thurs Feb 22			DNA ligation and bacterial transformation
1	5	Tues Feb 27			Examine candidate clones
2	1	Thurs Mar 1			Restriction map+ Article Discussion/Tissue Culture
2	2	Tues Mar			Lipofection
1	8	Thurs Mar			FACS analysis

2	1	Tues Mar			Tools for protein engineering
2	2	Thurs Mar			Assessing beta-galactosidase
2	3	Tues Mar			Purifying beta-galactosidase
2	4	Thurs Mar			Student presentations
		Spring Break Mar 27-31			No lab Mar 27-31

3	1	Tues Apr			Tools for systems engineering
3	2	Thurs Apr			Basic bacterial photography: black and white
3	3	Tues Apr			Advanced bacterial photography: 2 color
3	4	Thurs Apr			Device characterization
		Patriot's Day April 17-18			No lab Apr 18 or Apr 19
3	5	Thurs Apr			Measuring DNA, RNA, protein
3	6	Tues Apr			RT-PCR data analysis

4	1	Thurs Apr			Screening library
4	2	Tues May			Optimizing panning
4	3	Thurs May			Rescreening gold binders
4	4	Tues May			PCR of gold binding candidates
4	5	Thurs May			Student presentations
4	6	Tues May			Analyze sequence data
		Thurs May 18			Lecture only, no lab

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