Difference between revisions of "20.109(S24):Journal article presentation"

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#<font color = pink><b>[LP/WF/Pink]</b></font color>Chang, Z., ''et. al.'' "[[Media:Chang 2023 Pt-Se nanoprobes.pdf| Pt−Se-Bonded Nanoprobe for High-Fidelity Detection of Non-small Cell Lung Cancer and Enhancement of NIR II Photothermal Therapy.]]" (2023) Analytical Chemistry. https://doi.org/10.1021/acs.analchem.3c03511
 
#<font color = pink><b>[LP/WF/Pink]</b></font color>Chang, Z., ''et. al.'' "[[Media:Chang 2023 Pt-Se nanoprobes.pdf| Pt−Se-Bonded Nanoprobe for High-Fidelity Detection of Non-small Cell Lung Cancer and Enhancement of NIR II Photothermal Therapy.]]" (2023) Analytical Chemistry. https://doi.org/10.1021/acs.analchem.3c03511
 
#<font color = green><b>[KS/TR/Green]</b></font color>Chen, K., ''et. al.'' "[[Media:Chen 2024 Zn-Mg-CuMOF.pdf| Fabrication of a Nanoscale Magnesium/Copper Metal−Organic Framework on Zn-Based Guided Bone Generation Membranes for Enhancing Osteogenesis, Angiogenesis, and Bacteriostasis Properties.]]" (2024) Applied Materials & Interfaces. https://doi.org/10.1021/acsami.3c169703c03511
 
#<font color = green><b>[KS/TR/Green]</b></font color>Chen, K., ''et. al.'' "[[Media:Chen 2024 Zn-Mg-CuMOF.pdf| Fabrication of a Nanoscale Magnesium/Copper Metal−Organic Framework on Zn-Based Guided Bone Generation Membranes for Enhancing Osteogenesis, Angiogenesis, and Bacteriostasis Properties.]]" (2024) Applied Materials & Interfaces. https://doi.org/10.1021/acsami.3c169703c03511
#<font color = gold><b>[DH/TR/Yellow]</b></font color>Im, S.H., ''et. al.'' "[[Media:Im 2024 CRISPR-polymer Cu electrochemical sensor.pdf| A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance.]]" (2024) Applied Materials & Interfaces. https://doi.org/10.1021/acsami.3c17151
+
#<font color = gold><b>[DH/TR/Yellow]</b></font color>#<font color = teal><b>[SH/WF/Teal]</b></font color>Im, S.H., ''et. al.'' "[[Media:Im 2024 CRISPR-polymer Cu electrochemical sensor.pdf| A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance.]]" (2024) Applied Materials & Interfaces. https://doi.org/10.1021/acsami.3c17151
 
#Lee, H., ''et. al.'' "[[Media:Lee 2024 Ag skin electronics.pdf |Phase‑separated stretchable conductive nanocomposite to reduce contact resistance of skin electronics.]]" (2024) Scientific Reports. https://doi.org/10.1038/s41598-024-51980-1  
 
#Lee, H., ''et. al.'' "[[Media:Lee 2024 Ag skin electronics.pdf |Phase‑separated stretchable conductive nanocomposite to reduce contact resistance of skin electronics.]]" (2024) Scientific Reports. https://doi.org/10.1038/s41598-024-51980-1  
 
#<font color = orange><b>[SL/WF/Orange]</b></font color> Sanmugam, A., ''et. al.'' "[[Media:Sanmugam 2024 Ag wound healing.pdf |Fabrication of chitosan/fibrin-armored multifunctional silver nanocomposites to improve antibacterial and wound healing activities.]]" (2024) International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2023.128598  
 
#<font color = orange><b>[SL/WF/Orange]</b></font color> Sanmugam, A., ''et. al.'' "[[Media:Sanmugam 2024 Ag wound healing.pdf |Fabrication of chitosan/fibrin-armored multifunctional silver nanocomposites to improve antibacterial and wound healing activities.]]" (2024) International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2023.128598  

Revision as of 01:58, 15 March 2024

20.109(S24): Laboratory Fundamentals of Biological Engineering

Sp24 banner image.png

Spring 2024 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Overview and logistics

You will complete this assignment individually.

Please review the 20.109 statement on collaboration and integrity as you proceed.

In Module 1, you delivered a Research talk that was focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-reviewed and published. Reading, understanding, and explaining research related to your project are all important skills that will be important as you flex your scientist muscles.

As you prepare your talk be sure to review the resources provided on the Communication tab. In addition, please use the following link to view the full video from Susan McConnell: Designing effective scientific presentations. Lastly, review the Journal club presentation Evaluation rubric (linked here)!

Method of submission

Please submit your completed Journal club slides 1 hr prior to your scheduled laboratory session time to Canvas, with filename Name_LabSection_JC.pptx (for example, ImaStudent_TR_JC.pptx).

Length and format of presentations

You will have 10 minutes to discuss the journal article you select. It may be very difficult, or impossible, to discuss all of the figures within the article adequately in only 10 minutes. Therefore, this assignment is not only to present the work, but also to identify the data that is most important to the conclusions. It is also critical to consider how your presentation 'flows' from one experiment to the next. As when you write your own research, you want to deliver a coherent story during your journal presentation.

Format considerations

The timing provided here is a guideline for a 10-minute presentation. Your presentation may vary depending on the content.

Section Minutes Number of slides DO DON'T
Introduction ~2 2-3
  • Introduce the key concepts that the audience will need to follow your presentation.
  • Briefly state the overall scope and significance of the study -- what is the central question and why is it interesting?
  • Try to summarize background material with a model slide rather than lines of text. If text is needed, bring in the details as you speak using PowerPoint animation.
  • Don't assume you are addressing an expert audience.
  • Don't give more information than is absolutely needed to understand the rest of your talk.
  • Don't put too much information on each slide.
Data ~7 4-6
  • Present the data in a logical sequence, letting each slide build upon the previous ones.
  • Include a title for each slide. The title should be the conclusion and should be unique to the information on the slide.
  • Make every element of your slide visible to the entire room. This means 20-point font or greater.
  • Interpret each slide thoroughly and carefully.
  • Point out strengths and weaknesses of the data along the way.
  • Don't read your talk. Similarly, do not read lists from slides.
  • Don't put much information on each slide. Each slide should make only one point.
  • Never say, "I know you can't read this, but...". Everything on each slide should be legible.
  • Don't be afraid to remind the audience how the data fits into the overall question
Summary ~1 1
  • Review each of your main messages.
  • Clearly state what the study contributed to the field.
  • Don't repeat experimental details.
Question & Answer ? 0
  • Answer the question being asked. If you are unclear about the question, ask for clarification.
  • Respect every question and questioner.
  • Don't take too long with one question. If the discussion is involved, suggest meeting after the talk to discuss it more.

Helpful hints

  • A 10-minute talk is NOT a 30-minute talk given while racing through slides and speaking very quickly.
  • Consider ways to transition from one slide to the next to ensure the information is tied together.
  • Practice your presentation in front of people rather than in a room by yourself and practice several times!
  • Familiarize yourself with using a laser pointer and/or slide changer if you will use one during the actual presentation.
  • If you do choose to use a pointer, use it to direct attention to specific elements on the screen, rather than constantly gesturing in the general vicinity of your slide; otherwise, the audience will not know what's important. When you later make your own slides and figures, the apparent need for a pointer may actually mean you need to make a clearer slide.

Article selection

You may choose to select a journal article from those provided by the teaching faculty or you can select an article that is related to your Module 2 research from any peer-reviewed journal.

  • If you choose an article from below, please "reserve" it by putting your (initials/lab section/team color) next to the listing here.
    • For visibility, please use the following format to sign up if possible, substituting in your own initials and team color: <font color = purple><b>[IS/WF/Purple]</b></font color>, which will look like [IS/WF/Purple].
  • If you would like to discuss a paper not on the list below, please email it (as .pdf) to the teaching faculty (Noreen, Becky, and Jamie) with a brief description of the work.
  • The list of papers below is provided as a guideline for the types of papers that might be relevant for your presentation. You are not limited to the primary research articles on this list. The list is provided simply to give you an idea of the kinds of subjects that could make suitable presentations for the class. Feel free to search PubMed yourself to find articles of interest to you.

Please review the articles before making your final selection to ensure it is a paper that you find interesting and that you are comfortable presenting!

Bioremediation approaches

  1. Acosta-Luque, M., et. al. " Remediation of Pb‑contaminated soil using biochar‑based slow‑release P fertilizer and biomonitoring employing bioindicators." (2023) Scientific reports. https://doi.org/10.1038/s41598-022-27043-8
  2. [EW/TR/Pink] Howard, J., et. al. " Combating lead and cadmium exposure with an orally administered chitosan‑based chelating polymer." (2023) Scientific reports. https://doi.org/10.1038/s41598-023-28968-4
  3. [CK/TR/Orange] Ibuot, A., et. al. " Increased metal tolerance and bioaccumulation of zinc and cadmium in Chlamydomonas reinhardtii expressing a AtHMA4 C‐terminal domain protein.". (2020) Biotechnology and Bioengineering. DOI: 10.1002/bit.27476
  4. [AG/TR/Pink] Jia, X., et. al. " Display of lead‐binding proteins on Escherichia coli surface for lead bioremediation.". (2020) Biotechnology and Bioengineering. DOI: 10.1002/bit.27525
  5. [SL/TR/Blue] Li, J., et. al. " Efficient lead immobilization by bio-beads containing Pseudomonas rhodesiae and bone char.". (2023) Journal of Hazardous Materials. https://doi.org/10.1016/j.jhazmat.2023.130772
  6. Lone, S., et. al. " Gelatin–chitosan hydrogel particles for efficient removal of HgIJII) from wastewater.". (2019) Environ. Sci.: Water Res. Technol. DOI: 10.1039/c8ew00678d
  7. [SM/TR/Yellow]Lu, C., et. al. " Bioremediation potential of cadmium by recombinant Escherichia coli surface expressing metallothionein MTT5 from Tetrahymena thermophila.". (2023) Chemosphere. https://doi.org/10.1016/j.chemosphere.2022.136850
  8. Ma, H., et. al. " Bioremediation of cadmium polluted soil using a novel cadmium immobilizing plant growth promotion strain Bacillus sp. TZ5 loaded on biochar.". (2020) Journal of Hazardous Materials. https://doi.org/10.1016/j.jhazmat.2020.122065
  9. Mao, Q., et. al. " Indigenous cyanobacteria enhances remediation of arsenic-contaminated soils by regulating physicochemical properties, microbial community structure and function in soil microenvironment.". (2023) Science of the Total Environment. http://dx.doi.org/10.1016/j.scitotenv.2022.160543
  10. Sengupta, D., et. al. " Prospective bioremediation of toxic heavy metals in water by surfactant exopolysaccharide of Ochrobactrum pseudintermedium using cost‑effective substrate.". (2021) International Microbiology. https://doi.org/10.1007/s10123-021-00182-0
  11. Tripathi, S., et. al. " Elucidating the bioremediation mechanism of Scenedesmus sp. IITRIND2 under cadmium stress.". (2021) Chemosphere. https://doi.org/10.1016/j.chemosphere.2021.131196
  12. Wu, K., et. al. " Integrating FTIR 2D correlation analyses, regular and omics analyses studies on the interaction and algal toxicity mechanisms between graphene oxide and cadmium.". (2023) Journal of Hazardous Materials. https://doi.org/10.1016/j.jhazmat.2022.130298
  13. [NS/TR/Green] [VA/WF/Red] Wu, C., et. al. " Bioremediation of mercury-polluted soil and water by the plant symbiotic fungus Metarhizium robertsii.". (2022) PNAS. https://doi.org/10.1073/pnas.2214513119
  14. [LF/WF/Pink] Xue, Y., et. al. " Mercury bioremediation in aquatic environment by genetically modified bacteria with self-controlled biosecurity circuit.". (2022) Journal of Cleaner Production. https://doi.org/10.1016/j.jclepro.2022.130524
  15. Yuan, B., et. al. " Application of mixotrophic acidophiles for the bioremediation of cadmium-contaminated soils elevates cadmium removal, soil nutrient availability, and rice growth.". (2022). Ecotoxicology and Environmental Safety. https://doi.org/10.1016/j.ecoenv.2022.113499

Uses of heavy metals

  1. [JM/TR/Orange] Al-madani, H., et. al. " Quantification and biological evaluation of ZnxFe3 xO4 nanoparticle stiffness in a drug delivery system of MCF-7 cancer cells." (2024) Journal of Materials Chemistry B. DOI: 10.1039/d3tb02723f
  2. [LP/WF/Pink]Chang, Z., et. al. " Pt−Se-Bonded Nanoprobe for High-Fidelity Detection of Non-small Cell Lung Cancer and Enhancement of NIR II Photothermal Therapy." (2023) Analytical Chemistry. https://doi.org/10.1021/acs.analchem.3c03511
  3. [KS/TR/Green]Chen, K., et. al. " Fabrication of a Nanoscale Magnesium/Copper Metal−Organic Framework on Zn-Based Guided Bone Generation Membranes for Enhancing Osteogenesis, Angiogenesis, and Bacteriostasis Properties." (2024) Applied Materials & Interfaces. https://doi.org/10.1021/acsami.3c169703c03511
  4. [DH/TR/Yellow]#[SH/WF/Teal]Im, S.H., et. al. " A Wireless, CRISPR-Polymer Dot Electrochemical Sensor for the Diagnosis of Bacterial Pneumonia and Multi-Drug Resistance." (2024) Applied Materials & Interfaces. https://doi.org/10.1021/acsami.3c17151
  5. Lee, H., et. al. "Phase‑separated stretchable conductive nanocomposite to reduce contact resistance of skin electronics." (2024) Scientific Reports. https://doi.org/10.1038/s41598-024-51980-1
  6. [SL/WF/Orange] Sanmugam, A., et. al. "Fabrication of chitosan/fibrin-armored multifunctional silver nanocomposites to improve antibacterial and wound healing activities." (2024) International Journal of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2023.128598
  7. [TB/TR/Pink] [AL/WF/Yellow]Schwartz-Duval, A., et. al. "Intratumoral Biosynthesis of Gold Nanoclusters by Pancreatic Cancer to Overcome Delivery Barriers to Radiosensitization." (2024) ACS Nano. https://doi.org/10.1021/acsnano.3c04260
  8. Sheng, H., et. al. "A soft implantable energy supply system that integrates wireless charging and biodegradable Zn-ion hybrid supercapacitors." (2023) Science Advances. DOI: 10.1126/sciadv.adh80
  9. [HW/WF/Green]Wang, J., et. al. "CRISPR/Cas9-mediated SERS/colorimetric dual-mode lateral flow platform combined with smartphone for rapid and sensitive detection of Staphylococcus aureus." (2024) Biosensors and Bioelectronics. https://doi.org/10.1016/j.bios.2024.116046
  10. [NZ/WF/Yellow]Zare, A., et. al. "Label‑free electrochemical cancer cell detection leveraging hemoglobin‑encapsulated silver nanoclusters and Cu‑MOF nanohybrids on a graphene‑assisted dual‑modal probe." (2023) Scientific Reports. https://doi.org/10.1038/s41598-023-49418-1

Detection of heavy metals

  1. Bao, Q., et. al. " In situ detection of heavy metal ions in sewage with screen-printed electrode-based portable electrochemical sensors." (2023) Analyst. DOI: 10.1039/d1an01012c
  2. [HC/TR/Blue]Eom, H., et. al. " Improved toxicity analysis of heavy metal-contaminated water via a novel fermentative bacteria-based test kit." (2020) Chemosphere. https://doi.org/10.1016/j.chemosphere.2020.127412
  3. Gan, Y., et. al. " In-situ detection of cadmium with aptamer functionalized gold nanoparticles based on smartphone-based colorimetric system." (2020) Talenta. https://doi.org/10.1016/j.talanta.2019.120231
  4. Hasan, A., et. al. " Nanozyme-based sensing platforms for detection of toxic mercury ions: An alternative approach to conventional methods." (2020) Talenta. https://doi.org/10.1016/j.talanta.2020.120939
  5. Heidari, B., et. al. " Highly selective and sensitive recognition of multi‑ions in aqueous solution based on polymer‑grafted nanoparticle as visual colorimetric sensor." (2024) Scientific reports. https://doi.org/10.1038/s41598-023-50627-x
  6. [EJ/WF/Green]Jung, J., et. al. " Cell-free biosensors for rapid detection of water contaminants." (2020) Nature biotechnology. https://doi.org/10.1038/s41587-020-0571-7
  7. Liu, Y., et. al. " A gas reporting whole-cell microbial biosensor system for rapid on-site detection of mercury contamination in soils." (2020) Biosensors and Bioelectronics. https://doi.org/10.1016/j.bios.2020.112660
  8. Pan, S., et. al. " Electron microscopic imaging and NanoSIMS investigation on physiological responses of Aspergillus niger under Pb(II) and Cd(II) stress." (2023) Frontiers in Bioengineering and Biotechnology. DOI:10.3389/fbioe.2022.1096384
  9. Tzroya, A., et. al. " Optical Method for Detection and Classification of Heavy Metal Contaminants in Water Using Iso-pathlength Point Characterization." (2024) ACS Omega. https://doi.org/10.1021/acsomega.3c08792
  10. Wei, H., et. al. " Decoding the metabolic response of Escherichia coli for sensing trace heavy metals in water." (2022) PNAS. https://doi.org/10.1073/pnas.2210061120
  11. Yu, Y., et. al. " Bi‑functionality of glyoxal caged nucleic acid coupled with CRISPR/Cas12a system for Hg2+ determination." (2022) Microchimica Acta. https://doi.org/10.1007/s00604-024-06196-5