20.109(S21):Spring 2021 schedule

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20.109(S21): Laboratory Fundamentals of Biological Engineering

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Spring 2021 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Antibody engineering        M2: Drug discovery        M3: Protein engineering       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times:

  • Tuesday (T) and Thursday (R) 11 - 12 pm ([ Zoom access)]

Laboratory section times:

  • Tuesday (T) and Thursday (R) 1 - 5 pm ([ Zoom access])
  • Wednesday (W) and Friday (F) 1 - 5 pm ([ Zoom access])
MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 16/17 NLL
Slides
Orientation and laboratory tour
M1D1 R/F Feb 18/19 LM
In silico cloning library Orientation quiz
Homework due
M1D2 T/W Feb 23/24 LM
Enrich candidate clones from library using FACS
Homework due
M1D3 R/F Feb 25/26 LM
Harvest candidate clone plasmids from yeast library
Homework due
M1D4 T/W Mar 2/3 LM
Purify plasmids and sequence clones
Laboratory quiz
Homework due
M1D5 R/F Mar 4/5 LM
Analyze clone sequence and choose clone to characterize
Homework due
T/W Mar 9/10 Student holiday
M1D6 R/F Mar 11/12 LM
Characterize clone by ligand binding using flow cytometry
Homework due
M1D7 T/W Mar 16/17 LM
Analyze ligand titration curves
Laboratory quiz
Homework due
M2D1 R/F Mar 18/19 AK
Placeholder
Homework due
T/W Mar Mar 23/24 Student holiday
M2D2 R/F Mar 25/26 AK
Placeholder
Homework due
Data Summary draft due XXX at 10 pm
[ Blog post due] XXX at 10 pm
M2D3 T/W Mar 30/31 AK
Placeholder
Homework due
M2D4 R/F Apr 1/2 AK
Placeholder
Laboratory quiz
Homework due
Mini-presentation due XXX at 10 pm
T/W Apr 6/7 Journal club presentations
R/F Apr 8/9 Journal club presentations [ Blog post due] XXX at 10 pm
M2D5 T/W Apr 13/14 AK
Placeholder Homework due
M2D6 R/F Apr 15/16 AK
Placeholder
Homework due
T/W Apr 20/21 Student holiday
M2D7 R/F Apr 22/23 AK
Placeholder
Laboratory quiz
Homework due
M3D1 T/W Apr 27/28 AJ
Placeholder
Homework due
M3D2 R/F Apr 29/30 AJ
Placeholder
Homework due
| Research article due XXX at 10 pm
[ Blog post due] XXX at 10 pm
M3D3 T/W May 4/5 AJ
Placeholder
Laboratory quiz
Homework due
R/F May 6/7 Research proposal pitches
Develop Research proposal ideas and presentations
M3D4 T/W May 11/12 AJ
Placeholder
Homework due
M3D5 R/F May 13/14 AJ
Placeholder
Laboratory quiz
Homework due
T/W May 18/19 Research proposal presentations [ Blog post due] XXX at 10 pm
R May 20 Feedback session
Mini-report due XXX at 10 pm