Difference between revisions of "20.109(S21):Module 3"
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<font color = #0d368e>'''Research goal: Perform site-directed mutagenesis to alter the properties of a protein-based fluorescent sensor'''</font color> | <font color = #0d368e>'''Research goal: Perform site-directed mutagenesis to alter the properties of a protein-based fluorescent sensor'''</font color> | ||
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[[Image:Sp21 M3 overview v3.png|center|750px|thumb|Image generated using BioRender.]] | [[Image:Sp21 M3 overview v3.png|center|750px|thumb|Image generated using BioRender.]] | ||
Revision as of 14:48, 27 January 2021
Contents
Module 3
Lecturer: Prof. Alan Jasanoff
Instructors: Dr. Noreen Lyell, Dr. Leslie McClain, and Dr. Becky Meyer
TAs: Jeff Hsaio and Malek Kabani
Overview
Research goal: Perform site-directed mutagenesis to alter the properties of a protein-based fluorescent sensor
Lab links: day by day
M3D1: Review inverse pericam (IPC) cloning strategy
M3D2: Examine IPC mutations
M3D3: Induce and purify IPC variants
M3D4: Evaluate effect of mutations on IPC variants
M3D5: Design new IPC variant
Assignments
Research proposal presentation
Mini-report
