Difference between revisions of "20.109(S21):Module 3"

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<font color = #0d368e>'''Research goal: '''</font color>
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<font color = #0d368e>'''Research goal: Perform site-directed mutagenesis to alter the properties of a protein-based fluorescent sensor'''</font color>
  
  

Revision as of 14:21, 27 January 2021

20.109(S21): Laboratory Fundamentals of Biological Engineering

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Spring 2021 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Antibody engineering        M2: Drug discovery        M3: Protein engineering       


Module 3

Lecturer: Prof. Alan Jasanoff
Instructors: Dr. Noreen Lyell, Dr. Leslie McClain, and Dr. Becky Meyer
TAs: Jeff Hsaio and Malek Kabani

Overview

Research goal: Perform site-directed mutagenesis to alter the properties of a protein-based fluorescent sensor


Image generated using BioRender.


Lab links: day by day

M3D1: Review inverse pericam (IPC) cloning strategy
M3D2: Examine IPC mutations
M3D3: Induce and purify IPC variants
M3D4: Evaluate effect of mutations on IPC variants
M3D5: Design new IPC variant

Assignments

Research proposal presentation
Mini-report

References

Notes for teaching faculty

Prep notes for M3