Difference between revisions of "20.109(S21):Module 1"

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=<center>Module 3</center>=
=<center>Module 3</center>=
'''Lecturer:''' [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] <br>
'''Lecturer:''' [http://be.mit.edu/directory/leslie-mcclain Dr. Leslie McClain] <br>
'''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell] and [http://be.mit.edu/directory/leslie-mcclain Leslie McClain], and [http://be.mit.edu/directory/becky-meyer Becky Meyer]<br>
'''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Dr. Noreen Lyell] and [http://be.mit.edu/directory/leslie-mcclain Dr. Leslie McClain], and [http://be.mit.edu/directory/becky-meyer Dr. Becky Meyer]<br>
'''Research assistant:''' Sarah Cowles <br>
'''Research assistant:''' Sarah Cowles <br>

Revision as of 01:25, 23 January 2021

20.109(S21): Laboratory Fundamentals of Biological Engineering

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Spring 2021 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Antibody engineering        M2: Drug discovery        M3: Protein engineering       

Module 3

Lecturer: Dr. Leslie McClain
Instructors: Dr. Noreen Lyell and Dr. Leslie McClain, and Dr. Becky Meyer
Research assistant: Sarah Cowles

Engineering Antibodies Using Yeast Display

An antibody is a soluble or membrane bound protein, produced by immune cells in blood. In our bodies the purpose of an antibody is to recognize foreign substances, called antigens, and mark them for removal by cells that compromise our immune system. If one has a properly functioning immune system you've all been making new and improved antibodies your entire life.

Antibodies are also a critical reagent in medicine, research and diagnostics. Scientists have found ways to induce and purify antibodies from animals, and to also engineer cells to produce specific antibodies. In Mod1 we'll use a method developed in the Wittrup lab to screen a yeast library of antibodies, against a particular antigen, using Fluorescence Assisted Cell Sorting, and try to identify a single antibody that shows improved antigen binding in a quantitative binding assay.

This module was developed thanks to the invaluable help and support of Wittrup lab PhD student, Sarah Cowles, and the generous contribution of reagents from Prof. Wittrup.

Research goal:

Schematic overview of the experimental approach in Module 3

Lab links: day by day

M1D1: [[]]
M1D2: [[]]
M1D3: [[]]
M1D4: [[]]
M1D5: [[]]



Notes for teaching faculty

Prep notes for M3