20.109(S18):Class data

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20.109(S18): Laboratory Fundamentals of Biological Engineering

Sp18 banner image v2.png

Spring 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              


Module 1: Assessing ligand binding

M1D2: SMM analysis

GAL files linked here.

Scan images and set numbers assigned to each team:

Team Slide #1 Slide #2
T/R
Red 14399983, set 1 14400077, set 4
Orange 14400113, set 2 14400139, set 3
Yellow 14400114, set 2 14400140, set 3
Green 14399984, set 5 14399988, set 6
Blue 14400072, set 5 14399987, set 6
Pink 14399983, set 1 14400128, set 7
Purple 14400077, set 4 14399959, set 8
White 14400129, set 7 14400081, set 9
Grey 14400128, set 7 14399959, set 8
W/F
Red 14400129, set 7 14400081, set 9
Orange 14400128, set 7 14399959, set 8
Green 14400143, set 11 14399950, set 12
Blue 14400147, set 10 14399951, set 12
Pink 14400146, set 10 14399947, set 11
Purple 14399983, set 1 14400077, set 4
White 14400113, set 2 14400139, set 3


Chosen Compounds

List of Chosen Compounds
List of Compounds with SMILES

M1D4: Protein Gel Images

Team gel image
T/R
Red Sp18_TRred
Orange Sp18_TRorange
Yellow Sp18_TRyellow
Green Sp18_TRgreen
Blue Sp18_TRblue
Pink Sp18_TRpink
Purple Sp18_TRpurple
White Sp18_TRwhite
Grey Sp18_TRgrey
W/F
Red Sp18_WFred
Orange Sp18_WForange
Green Sp18_WFgreen
Blue Sp18_WFblue
Pink Sp18_WFpink
Purple Sp18_WFpurple
Gray Sp18_WFgray


M1D5: PPIase Assay Results

TR Raw Data
Sp18_PPIase_TR_Run1
Sp18_PPIase_TR_Run2
Sp18_PPIase_TR_Run3

WF Raw Data
Sp18_PPIase_WF_Run1
Sp18_PPIase_WF_Run2
Sp18_PPIase_WF_Run3

Pooled PPIase Data
TR & WF pooled Conditions #1, #2, #3, #6, and #7


Team PPIase Assay Results Analyzed Results
T/R
Red Sp18_TRred_PPIase Sp18_TRred_PPIase_Analyzed
Orange Sp18_TRorange_PPIase Sp18_TRorange_PPIase_Analyzed
Yellow Sp18_TRyellow_PPIase Sp18_TRyellow_PPIase_Analyzed
Green Sp18_TRgreen_PPIase Sp18_TRgreen_PPIase_Analyzed
Blue Sp18_TRblue_PPIase Sp18_TRblue_PPIase_Analyzed
Pink Sp18_TRpink_PPIase Sp18_TRpink_PPIase_Analyzed
Purple Sp18_TRpurple_PPIase Sp18_TRpurple_PPIase_Analyzed
White Sp18_TRwhite_PPIase Sp18_TRwhite_PPIase_Analyzed
Grey Sp18_TRgrey_PPIase Sp18_TRgray_PPIase_Analyzed
W/F
Red Sp18_WFred_PPIase Sp18_WFred_PPIaseAnalyzed
Orange Sp18_WForange_PPIase Sp18_WForange_PPIaseAnalyzed
Green Sp18_WFgreen_PPIase Sp18_WFgreen_PPIaseAnalyzed
Blue Sp18_WFblue_PPIase Sp18_WFblue_PPIaseAnalyzed
Pink Sp18_WFpink_PPIase Sp18_WFpink_PPIaseAnalyzed
Purple Sp18_WFpurple_PPIase Sp18_WFpurple_PPIaseAnalyzed
Gray Sp18_WFgray_PPIase Sp18_WFgray_PPIaseAnalyzed

M1D6: DSF Assay Results

Rapamycin Concentrations

Sp18 rapamycinconcentratoins.png


TR DSF Raw Data
Sp18_TR_DSF_PlateMap
Sp18_TR_FKBP12_Melt Curve RFU Results
Sp18_TR_FKBP12_Melt Curve Derivative Results

WF DSF Raw Data
Sp18_WF_DSF_PlateMap
Sp18_WF_FKBP12_Melt Curve RFU Results
Sp18_WF_FKBP12_Melt Curve Derivative Results



Excel file combining DSF derivative data FKBP12 with different concentrations of Rapamycin




Module 2: Measuring gene expression

M2D9: Cell viability data

==Module 3: Engineering biomaterials==