Difference between revisions of "20.109(S18):Class data"

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[[Media: Sp18_ChosenCompounds.pdf|List of Chosen Compounds]]<br>
 
[[Media: Sp18_ChosenCompounds.pdf|List of Chosen Compounds]]<br>
 
[[Media:Sp18 FKBP12 unique hits SMILES.xlsx|List of Compounds with SMILES]]<br>
 
[[Media:Sp18 FKBP12 unique hits SMILES.xlsx|List of Compounds with SMILES]]<br>
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[[Media:Sp18 FKBP12 unique hits SMILESwithTeams.xlsx|List of Compounds with SMILES and Teams]]<br>
  
 
===M1D4: Protein Gel Images===
 
===M1D4: Protein Gel Images===

Revision as of 17:27, 10 March 2018

20.109(S18): Laboratory Fundamentals of Biological Engineering

Sp18 banner image v2.png

Spring 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              


Module 1: Assessing ligand binding

M1D2: SMM analysis

GAL files linked here.

Scan images and set numbers assigned to each team:

Team Slide #1 Slide #2
T/R
Red 14399983, set 1 14400077, set 4
Orange 14400113, set 2 14400139, set 3
Yellow 14400114, set 2 14400140, set 3
Green 14399984, set 5 14399988, set 6
Blue 14400072, set 5 14399987, set 6
Pink 14399983, set 1 14400128, set 7
Purple 14400077, set 4 14399959, set 8
White 14400129, set 7 14400081, set 9
Grey 14400128, set 7 14399959, set 8
W/F
Red 14400129, set 7 14400081, set 9
Orange 14400128, set 7 14399959, set 8
Green 14400143, set 11 14399950, set 12
Blue 14400147, set 10 14399951, set 12
Pink 14400146, set 10 14399947, set 11
Purple 14399983, set 1 14400077, set 4
White 14400113, set 2 14400139, set 3


Chosen Compounds

List of Chosen Compounds
List of Compounds with SMILES
List of Compounds with SMILES and Teams

M1D4: Protein Gel Images

Team gel image
T/R
Red Sp18_TRred
Orange Sp18_TRorange
Yellow Sp18_TRyellow
Green Sp18_TRgreen
Blue Sp18_TRblue
Pink Sp18_TRpink
Purple Sp18_TRpurple
White Sp18_TRwhite
Grey Sp18_TRgrey
W/F
Red Sp18_WFred
Orange Sp18_WForange
Green Sp18_WFgreen
Blue Sp18_WFblue
Pink Sp18_WFpink
Purple Sp18_WFpurple
Gray Sp18_WFgray


M1D5: PPIase Assay Results

TR Raw Data
Sp18_PPIase_TR_Run1
Sp18_PPIase_TR_Run2
Sp18_PPIase_TR_Run3

WF Raw Data
Sp18_PPIase_WF_Run1
Sp18_PPIase_WF_Run2
Sp18_PPIase_WF_Run3

Pooled PPIase Data
TR & WF pooled Conditions #1, #2, #3, #6, and #7


Team PPIase Assay Results
T/R
Red Sp18_TRred_PPIase
Orange Sp18_TRorange_PPIase
Yellow Sp18_TRyellow_PPIase
Green Sp18_TRgreen_PPIase
Blue Sp18_TRblue_PPIase
Pink Sp18_TRpink_PPIase
Purple Sp18_TRpurple_PPIase
White Sp18_TRwhite_PPIase
Grey Sp18_TRgrey_PPIase
W/F
Red Sp18_WFred_PPIase
Orange Sp18_WForange_PPIase
Green Sp18_WFgreen_PPIase
Blue Sp18_WFblue_PPIase
Pink Sp18_WFpink_PPIase
Purple Sp18_WFpurple_PPIase
Gray Sp18_WFgray_PPIase


M1D6: DSF Assay Results

Rapamycin Concentrations

Sp18 rapamycinconcentratoins.png


TR DSF Raw Data
Sp18_TR_DSF_PlateMap
Sp18_TR_FKBP12_Melt Curve RFU Results
Sp18_TR_FKBP12_Melt Curve Derivative Results

WF DSF Raw Data
Sp18_WF_DSF_PlateMap
Sp18_WF_FKBP12_Melt Curve RFU Results
Sp18_WF_FKBP12_Melt Curve Derivative Results



Excel file combining DSF derivative data FKBP12 with different concentrations of Rapamycin




Team DSF Assay Results
T/R
Red Sp18_TRred_DSF
Orange Sp18_TRorange_DSF
Yellow Sp18_TRyellow_DSF
Green Sp18_TRgreen_DSF.
Blue Sp18_TRblue_DSF.
Pink Sp18_TRpink_DSF
Purple Sp18_TRpurple_DSF
White Sp18_TRwhite_DSF
Grey Sp18_TRgrey_DSF
W/F
Red Sp18_WFred_DSF
Orange Sp18_WForange_DSF
Green Sp18_WFgreen_DSF
Blue Sp18_WFblue_DSF
Pink Sp18_WFpink_DSF
Purple Sp18_WFpurple_DSF
Gray Sp18_WFgray_DSF

Module 2: Measuring gene expression

M2D9: Cell viability data

==Module 3: Engineering biomaterials==