Difference between revisions of "20.109(S18):Analyze small microarray data and induce protein expression (Day2)"

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Revision as of 16:03, 4 January 2018

20.109(S18): Laboratory Fundamentals of Biological Engineering

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Spring 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Assessing ligand binding        2. Measuring gene expression        3. Engineering biomaterials              

Part 4: Induce FKBP12 protein expression

  1. Add IPTG to a final concentration of 1 mM to your E. coli bacterial culture, and return the flask to the 25 °C water bath shaker.
Your culture will incubate for ~16 hr in the water bath then the teaching faculty will collect the cells by centrifugation at 3000 g for 10 min. The harvested cells will be stored in the -80 °C freezer.
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