Difference between revisions of "20.109(S17): TA notes for orientation"

From Course Wiki
Jump to: navigation, search
(Station 8: lab math)
(Station 5: lab safety)
 
(3 intermediate revisions by 2 users not shown)
Line 4: Line 4:
 
==Station 1: pipetting==
 
==Station 1: pipetting==
  
Prepare at TA bench:
+
Prepare at instructors bench:
*pipet tips
+
*cuvette boxes out
*P1000 and P200
+
*25 mL of DI water in conical tubes (students can refill from sink)
*cuvettes
+
*~30 mL XC 0.01% aliquoted in conical tubes
*2x 50 mL of DI water in conical tubes
+
*1x 13 mL XC 0.01% in conical tubes
+
 
Each group uses:
 
Each group uses:
 
*~10 mL water
 
*~10 mL water
*~ mL XC 0.01%
+
*~4 mL XC 0.01%
 
*10 non-UV cuvettes
 
*10 non-UV cuvettes
  
Line 37: Line 35:
  
 
Wipe down the equipment and the sample card with a damp paper towel to rid dust.
 
Wipe down the equipment and the sample card with a damp paper towel to rid dust.
 
==Station 5: TC room==
 
 
Post cards with famous scientist names on the equipment indicated.
 
  
 
==Station 5: lab safety==
 
==Station 5: lab safety==
  
Be prepared to give hints about where to find the safety shower, fire blanket, and spill kits.
+
Be prepared to give hints about where to find the safety shower, chemical waste, and spill kits.
  
 
==Station 6: graph data in Excel==
 
==Station 6: graph data in Excel==
Line 53: Line 47:
  
 
Be prepared to answer questions about this version of basic lab math.
 
Be prepared to answer questions about this version of basic lab math.
 +
 +
==Station 8: TC room==
 +
 +
Post cards with famous scientist names on the equipment indicated.

Latest revision as of 16:07, 6 September 2017

20.109(S17): Laboratory Fundamentals of Biological Engineering

KoehlerDotBanner.png

Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

Station 1: pipetting

Prepare at instructors bench:

  • cuvette boxes out
  • 25 mL of DI water in conical tubes (students can refill from sink)
  • ~30 mL XC 0.01% aliquoted in conical tubes

Each group uses:

  • ~10 mL water
  • ~4 mL XC 0.01%
  • 10 non-UV cuvettes

Station 2: making solutions

At the balance:

  • Dextrose in 50 mL conical tubes, ~ 3/4 full, 3 of them
  • Check weigh boat and stir bar stocks, wash spatulas

All across from Yellow group bench:

  • 8x 50 mL conical tubes
  • 2x small graduated cylinders (100 mL)
  • 2x 0.5 L media bottle filled with distilled water, 2 sets only
  • 8x 25 mL pipets, 1 per team plus 2 extra
  • 2x pipet bulbs, 2 only

Station 3: spectrophotometer

Specs should be turned on at the beginning of lab. Make sure the DU 730 wavelength scan is set to read between 300 and 900 nm. Prepare one set of reference solutions (prepared according to station 1), including a blank, and leave them on top of the DU 640.

Station 4: gel reading

Wipe down the equipment and the sample card with a damp paper towel to rid dust.

Station 5: lab safety

Be prepared to give hints about where to find the safety shower, chemical waste, and spill kits.

Station 6: graph data in Excel

Be prepared to answer questions about this version of Excel.

Station 7: lab math

Be prepared to answer questions about this version of basic lab math.

Station 8: TC room

Post cards with famous scientist names on the equipment indicated.