20.109(S17): Spring 2017 schedule
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (4-149)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)
| MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
| T/W Feb 7/8 | NLL | Orientation | ||||
| 1 | 1 | R/F Feb 9/10 | AK | In silico cloning and induce protein expression | Orientation quiz | |
| 1 | 2 | T/W Feb 14/15 | AK | Purify induced protein | ||
| 1 | 3 | R/F Feb 16/17 | AK | Evaluate purity and concentration of protein | Laboratory quiz | |
| T/W Feb 21/22 | President's day holiday | |||||
| 1 | 4 | R/F Feb 23/24 | AK | Screen ligand library for FKBP12 binders | ||
| 1 | 5 | T/W Feb/Mar 28/1 | SD | Scan slides to identify FKBP12 binders | Laboratory quiz | |
| 1 | 6 | R/F Mar 2/3 | AK | Complete data analysis | ||
| 1 | 7 | T/W Mar 7/8 | AK | Identify chemical structures common among FKBP12 binders | Laboratory quiz | |
| 2 | 1 | R/F Mar 9/10 | LS | Confirm cell lines and practice tissue culture techniques | ||
| 2 | 2 | T/W Mar 14/15 | LS | Complete Western blot and induce DNA damage for survival and quantitative PCR assays | Laboratory quiz Draft due Mar 13 at 10pm | |
| 2 | 3 | R/F Mar 16/17 | LS | Assess cell survival and harvest RNA for quantitative PCR assay | Mini presentation March 18 at 10 pm | |
| 2 | 4 | T/W Mar 21/22 | LS | Examine transcript levels in response to DNA damage | Laboratory quiz | |
| 2 | 5 | R/F Mar 23/24 | LS | Journal club I | ||
| T/W Mar 28/29 | Spring vacation | Revision due March 27 at 10pm | ||||
| R/F Mar 30/31 | Spring vacation | |||||
| 2 | 6 | T/W Apr 4/5 | EF | D6 | ||
| 2 | 7 | R/F Apr 6/7 | EF | D7 | ||
| 2 | 8 | T/W Apr 11/12 | LS | Journal club II | ||
| 2 | 9 | R/F Apr 13/14 | LS | D9 | Laboratory quiz | |
| T/W Apr 18/19 | Patriot's day holiday | |||||
| 3 | 1 | R/F Apr 20/21 | AB | Grow phage active material | Research article due April 22 at 10 pm | |
| 3 | 2 | T/W Apr 25/26 | AB | Purify active material | Laboratory quiz | |
| 3 | 3 | R/F Apr 27/28 | Construct cathode | |||
| 3 | T/W May 2/3 | AB | Lecture, but no laboratory | |||
| 3 | 4 | R/F May 4/5 | AB | Visualize phage nanowires | ||
| 3 | 5 | T/W May 9/10 | AB | Assemble and test battery | Laboratory quiz | |
| R/F May 11/12 | Research proposal presentations | |||||
| T/W May 16/17 | Open Lab | Mini report due May 16 / 17 at 10 pm | ||||
| R May 18 | Feedback and celebratory lunch | |||||
