Difference between revisions of "20.109(S17): Spring 2017 schedule"
From Course Wiki
Noreen Lyell (Talk | contribs) |
Noreen Lyell (Talk | contribs) |
||
| Line 102: | Line 102: | ||
| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| Assess cell survival and harvest RNA for quantitative PCR assay | | Assess cell survival and harvest RNA for quantitative PCR assay | ||
| − | | [[20.109(S17): M1 Mini-presentation| Mini presentation due]] Sat, Mar 18 at 10 pm | + | | [[20.109(S17):Homework#Due_M2D3| Homework due]] <br> [[20.109(S17): M1 Mini-presentation| Mini presentation due]] Sat, Mar 18 at 10 pm |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 109: | Line 109: | ||
| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| Examine transcript levels in response to DNA damage | | Examine transcript levels in response to DNA damage | ||
| − | | <font color=#04B45F>'''Laboratory quiz'''</font color> | + | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D4| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 123: | Line 123: | ||
| | | | ||
| <font color = 999999>Spring vacation</font color> | | <font color = 999999>Spring vacation</font color> | ||
| − | | | + | | [[20.109(S17): M1 Data Summary| Data Summary revision due]] Mon, Mar 27 at 10 pm |
|-- | |-- | ||
| | | | ||
| Line 136: | Line 136: | ||
| T/W Apr 4/5 | | T/W Apr 4/5 | ||
| [http://be.mit.edu/directory/ernest-fraenkel EF] | | [http://be.mit.edu/directory/ernest-fraenkel EF] | ||
| − | | | + | | Practice data analysis methods |
| − | | | + | | [[20.109(S17):Homework#Due_M2D6| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 143: | Line 143: | ||
| R/F Apr 6/7 | | R/F Apr 6/7 | ||
| [http://be.mit.edu/directory/ernest-fraenkel EF] | | [http://be.mit.edu/directory/ernest-fraenkel EF] | ||
| − | | | + | | Complete data analysis |
| − | | | + | | [[20.109(S17):Homework#Due_M2D7| Homework due]] |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 151: | Line 151: | ||
| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| [[20.109(S17):Journal Club II (Day8)| Journal club II]] | | [[20.109(S17):Journal Club II (Day8)| Journal club II]] | ||
| − | | | + | | |
|-- | |-- | ||
| 2 | | 2 | ||
| Line 157: | Line 157: | ||
| R/F Apr 13/14 | | R/F Apr 13/14 | ||
| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| − | | | + | | Examine quantitative PCR results |
| − | | <font color=#04B45F>'''Laboratory quiz'''</font color> | + | | <font color=#04B45F>'''Laboratory quiz'''</font color> <br> [[20.109(S17):Homework#Due_M2D9| Homework due]] |
|-- | |-- | ||
| colspan="7" | | | colspan="7" | | ||
Revision as of 21:20, 30 January 2017
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (4-149)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)
| MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
| T/W Feb 7/8 | NLL | Orientation | ||||
| 1 | 1 | R/F Feb 9/10 | AK | In silico cloning and induce protein expression | Orientation quiz Homework due | |
| 1 | 2 | T/W Feb 14/15 | AK | Purify induced protein | Homework due | |
| 1 | 3 | R/F Feb 16/17 | AK | Evaluate purity and concentration of protein | Laboratory quiz Homework due | |
| T/W Feb 21/22 | President's day holiday | |||||
| 1 | 4 | R/F Feb 23/24 | AK | Screen ligand library for FKBP12 binders | Homework due | |
| 1 | 5 | T/W Feb/Mar 28/1 | SD | Scan slides to identify FKBP12 binders | Laboratory quiz Homework due | |
| 1 | 6 | R/F Mar 2/3 | AK | Complete data analysis | Homework due | |
| 1 | 7 | T/W Mar 7/8 | AK | Identify chemical structures common among FKBP12 binders | Laboratory quiz Homework due | |
| 2 | 1 | R/F Mar 9/10 | LS | Confirm cell lines and practice tissue culture techniques | Homework due | |
| 2 | 2 | T/W Mar 14/15 | LS | Complete Western blot and induce DNA damage for survival and quantitative PCR assays | Laboratory quiz Homework due Data Summary draft due Mon, Mar 13 at 10 pm | |
| 2 | 3 | R/F Mar 16/17 | LS | Assess cell survival and harvest RNA for quantitative PCR assay | Homework due Mini presentation due Sat, Mar 18 at 10 pm | |
| 2 | 4 | T/W Mar 21/22 | LS | Examine transcript levels in response to DNA damage | Laboratory quiz Homework due | |
| 2 | 5 | R/F Mar 23/24 | LS | Journal club I | ||
| T/W Mar 28/29 | Spring vacation | Data Summary revision due Mon, Mar 27 at 10 pm | ||||
| R/F Mar 30/31 | Spring vacation | |||||
| 2 | 6 | T/W Apr 4/5 | EF | Practice data analysis methods | Homework due | |
| 2 | 7 | R/F Apr 6/7 | EF | Complete data analysis | Homework due | |
| 2 | 8 | T/W Apr 11/12 | LS | Journal club II | ||
| 2 | 9 | R/F Apr 13/14 | LS | Examine quantitative PCR results | Laboratory quiz Homework due | |
| T/W Apr 18/19 | Patriot's day holiday | |||||
| 3 | 1 | R/F Apr 20/21 | AB | Grow phage active material | Research article due April 22 at 10 pm | |
| 3 | 2 | T/W Apr 25/26 | AB | Purify active material | Laboratory quiz | |
| 3 | 3 | R/F Apr 27/28 | Construct cathode | |||
| 3 | T/W May 2/3 | AB | Lecture, but no laboratory | |||
| 3 | 4 | R/F May 4/5 | AB | Visualize phage nanowires | ||
| 3 | 5 | T/W May 9/10 | AB | Assemble and test battery | Laboratory quiz | |
| R/F May 11/12 | Research proposal presentations | |||||
| T/W May 16/17 | Open Lab | Mini report due May 16 / 17 at 10 pm | ||||
| R May 18 | Feedback and celebratory lunch | |||||
