Difference between revisions of "20.109(S17): Spring 2017 schedule"
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| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| Complete Western blot and induce DNA damage for survival and quantitative PCR assays | | Complete Western blot and induce DNA damage for survival and quantitative PCR assays | ||
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| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| Examine transcript levels in response to DNA damage | | Examine transcript levels in response to DNA damage | ||
| − | | | + | | <font color=#04B45F>'''Laboratory quiz'''</font color> |
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| [http://be.mit.edu/directory/leona-d-samson LS] | | [http://be.mit.edu/directory/leona-d-samson LS] | ||
| D9 | | D9 | ||
| − | | | + | | <font color=#04B45F>'''Laboratory quiz'''</font color> |
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| colspan="7" | | | colspan="7" | | ||
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| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
| [[20.109(S17):Purify active materials (Day2) | Purify active material]] | | [[20.109(S17):Purify active materials (Day2) | Purify active material]] | ||
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| [http://be.mit.edu/directory/angela-belcher AB] | | [http://be.mit.edu/directory/angela-belcher AB] | ||
| [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]] | | [[20.109(S17):Battery assembly and testing (Day5) | Assemble and test battery ]] | ||
| − | | | + | | <font color=#04B45F>'''Laboratory quiz'''</font color> |
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Revision as of 20:25, 27 January 2017
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (4-149)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)
| MODULE | DAY | DATE | LECTURER | LABORATORY EXPERIMENTS | ASSIGNMENTS | |
| T/W Feb 7/8 | NLL | Orientation | ||||
| 1 | 1 | R/F Feb 9/10 | AK | In silico cloning and induce protein expression | Orientation quiz | |
| 1 | 2 | T/W Feb 14/15 | AK | Purify induced protein | ||
| 1 | 3 | R/F Feb 16/17 | AK | Evaluate purity and concentration of protein | Laboratory quiz | |
| T/W Feb 21/22 | President's day holiday | |||||
| 1 | 4 | R/F Feb 23/24 | AK | Screen chemical library for FKBP12 binders | ||
| 1 | 5 | T/W Feb/Mar 28/1 | SD | Scan slides to identify FKBP12 binders | Laboratory quiz | |
| 1 | 6 | R/F Mar 2/3 | AK | Complete data analysis | ||
| 1 | 7 | T/W Mar 7/8 | AK | Identify chemical structures common among FKBP12 binders | Laboratory quiz | |
| 2 | 1 | R/F Mar 9/10 | LS | Confirm cell lines and practice tissue culture techniques | ||
| 2 | 2 | T/W Mar 14/15 | LS | Complete Western blot and induce DNA damage for survival and quantitative PCR assays | Laboratory quiz | |
| 2 | 3 | R/F Mar 16/17 | LS | Assess cell survival and harvest RNA for quantitative PCR assay | ||
| 2 | 4 | T/W Mar 21/22 | LS | Examine transcript levels in response to DNA damage | Laboratory quiz | |
| 2 | 5 | R/F Mar 23/24 | LS | Journal club I | ||
| T/W Mar 28/29 | Spring vacation | |||||
| R/F Mar 30/31 | Spring vacation | |||||
| 2 | 6 | T/W Apr 4/5 | EF | D6 | ||
| 2 | 7 | R/F Apr 6/7 | EF | D7 | ||
| 2 | 8 | T/W Apr 11/12 | LS | Journal club II | ||
| 2 | 9 | R/F Apr 13/14 | LS | D9 | Laboratory quiz | |
| T/W Apr 18/19 | Patriot's day holiday | |||||
| 3 | 1 | R/F Apr 20/21 | AB | Grow phage active material | ||
| 3 | 2 | T/W Apr 25/26 | AB | Purify active material | Laboratory quiz | |
| R/F Apr 27/28 | Lecture, but no laboratory | |||||
| 3 | 3 | T/W May 2/3 | AB | Construct cathode | ||
| 3 | 4 | R/F May 4/5 | AB | Visualize phage nanowires | ||
| 3 | 5 | T/W May 9/10 | AB | Assemble and test battery | Laboratory quiz | |
| R/F May 11/12 | Research proposal presentations | |||||
| R May 18 | Feedback and celebratory lunch | |||||
