Difference between revisions of "20.109(S14): TA notes for module 2"
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==General notes== | ==General notes== | ||
| − | <font color=red> | + | <font color=red> I need to figure out the quiz dates.</font color> |
See also GoogleDoc for aliquoting amounts. | See also GoogleDoc for aliquoting amounts. | ||
'''Scheme:''' | '''Scheme:''' | ||
| + | -Plate wild type K1 and Ku80 deficient xrs6 cells | ||
| + | -Collect lysates for Westerns to measure Ku80 | ||
| + | -Prepare damaged DNA plasmids of varying topologies | ||
| + | -Transfect K1 and xrs6 cells with damaged DNA | ||
| + | -Measure DNA damage repair via NHEJ by flow cytometry | ||
'''Key preparation:''' | '''Key preparation:''' | ||
| + | Note to TA: | ||
* While you read the wiki, test each link, and make any link descriptions bold that are not already. | * While you read the wiki, test each link, and make any link descriptions bold that are not already. | ||
* As you read the protocols, add reagent calculations to the GoogleDoc as needed. | * As you read the protocols, add reagent calculations to the GoogleDoc as needed. | ||
Revision as of 04:54, 12 March 2014
Contents
General notes
I need to figure out the quiz dates.
See also GoogleDoc for aliquoting amounts.
Scheme: -Plate wild type K1 and Ku80 deficient xrs6 cells -Collect lysates for Westerns to measure Ku80 -Prepare damaged DNA plasmids of varying topologies -Transfect K1 and xrs6 cells with damaged DNA -Measure DNA damage repair via NHEJ by flow cytometry
Key preparation:
Note to TA:
- While you read the wiki, test each link, and make any link descriptions bold that are not already.
- As you read the protocols, add reagent calculations to the GoogleDoc as needed.
Day-by-day
Day 1
Materials required: None
Day of Lab (T/W):
After Lab:
How it went:
Day 2
Materials required: Please read as much as you can of Parts 1-3 and create an automated worksheet (e.g., in Excel) that will perform the required calculations for the day. A partially prepared worksheet is linked here. We will NOT collect your final worksheet this time; it is only for your benefit.
Day of Lab (R/F):
How it went:
Day 3
Materials required:
Day of Lab (T/W):
After Lab:
How it went:
Day 4
Materials required:
Day of Lab (R/F):
After Lab:
How it went:
Day 5
Materials required:
Day of Lab (T/W):
After Lab:
How it went:
Day 6
Materials required:
Day of Lab (R/F):
After Lab:
How it went:
Day 7
Materials required:
Day of Lab (T/W):
After Lab:
How it went:
Day 8
Materials required:
Day of Lab (R/F):
After Lab:
How it went: