Difference between revisions of "20.109(S13): TA notes for module 1"
From Course Wiki
(→Day 2) |
(→Day 2) |
||
| Line 29: | Line 29: | ||
'''Materials required:''' | '''Materials required:''' | ||
*Bird samples! At 100 μL each in a '''2 mL''' tube | *Bird samples! At 100 μL each in a '''2 mL''' tube | ||
| − | **Use filter pipet tip to transfer -- consult Agi about which samples to prepare | + | **Use filter pipet tip to transfer and store at -80 °C -- consult Agi about which samples to prepare |
*Aliquots of components from QiaAMP stool kit (see GoogleDoc) | *Aliquots of components from QiaAMP stool kit (see GoogleDoc) | ||
**Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early | **Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early | ||
| Line 37: | Line 37: | ||
'''Day of Lab (R/F):''' | '''Day of Lab (R/F):''' | ||
| + | *Thaw that day's 100 μL samples in the fridge starting just before lecture. -Agi will do | ||
| + | *Place 4 ice buckets around the room (between pairs) for keeping samples chilled at first. | ||
| + | *Before lab, move samples to the ice bucket nearest the pair that will be using those particular samples (see table on Talk page, ready shortly). | ||
*Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench | *Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench | ||
*Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C | *Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C | ||
| − | *Either set up second heat block at 56 °C or turn on the small oven (may be easiest) | + | *Either set up second heat block at 56 °C or turn on the small oven (may be easiest). -Agi will do |
'''After Lab:''' | '''After Lab:''' | ||
Revision as of 15:11, 12 February 2013
Coming soon!
Contents
General notes
See also GoogleDoc for aliquoting amounts.
Scheme:
Key preparation:
Day-by-day
Day 1
Materials required:
Day of Lab (R/F):
After Lab:
How it went: Pre-lab ran longer than it should have, leaving students with just over two hours to complete their designs -- not enough time for most.
Day 2
Materials required:
- Bird samples! At 100 μL each in a 2 mL tube
- Use filter pipet tip to transfer and store at -80 °C -- consult Agi about which samples to prepare
- Aliquots of components from QiaAMP stool kit (see GoogleDoc)
- Do NOT put out ethanol until after everyone is at the incubation step; we don't want them to accidentally add it too early
- Some aliquots may be approximate (use your judgment). For example, it will be easier to pipet approximately 0.5 mL into each tube using a serological pipet than to pipet exactly 0.46 mL eight times per day, times several reagents!
- ASL and AL must be aliquoted the day of lab (see below)
- InhibiTEX tablets should be singly available -- pre-cut around them; otherwise it is too easy to push out two instead of one
Day of Lab (R/F):
- Thaw that day's 100 μL samples in the fridge starting just before lecture. -Agi will do
- Place 4 ice buckets around the room (between pairs) for keeping samples chilled at first.
- Before lab, move samples to the ice bucket nearest the pair that will be using those particular samples (see table on Talk page, ready shortly).
- Check ASL and AL for precipitates; pre-heat at about 40-50 °C in the water bath at the TA bench
- Set up the heat block at the front bench. Fill tube slots with water and set at 70 °C
- Either set up second heat block at 56 °C or turn on the small oven (may be easiest). -Agi will do
After Lab:
- Store extraction samples at -20 °C until next time
