Difference between revisions of "20.109(S08):Module 2"
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(New page: Module 2 Day 1: Start-up protein engineering<br> (Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?...) |
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− | + | <div style="padding: 10px; width: 640px; border: 5px solid #FFCC33;"> | |
− | + | ==Module 2== | |
− | [[ | + | '''Instructors:''' [http://web.mit.edu/be/people/jasanoff.htm Alan Jasanoff] and [http://openwetware.org/wiki/User:AgiStachowiak | Agi Stachowiak] |
− | + | '''TA:''' [http://openwetware.org/wiki/User:Victor_S._Lelyveld |Victor Lelyveld] | |
− | [ | + | In this experiment, you will modify a protein called inverse pericam (developed by [http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=11248055&ordinalpos=5&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Nagai et al.]) in order to change its fluorescence properties. Inverse pericam (IPC) comprises a permuted fluorescent protein linked to a calcium sensor. The inverse in the name refers to the fact that this protein shines brightly in the absence of calcium, but dimly once calcium is added. The dissociation constant <math>K_D</math> of wild-type IPC with respect to calcium is reported to be 0.2 |