Difference between revisions of "20.109(S08):Module 2"
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(Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?) | (Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?) | ||
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| + | (Students dissect pericam into constituent parts today or next time?) | ||
[[20.109(S08):Design protein modification | Module 2 Day 2: Design protein modification]]<br> | [[20.109(S08):Design protein modification | Module 2 Day 2: Design protein modification]]<br> | ||
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(Also lipofect wild-type plasmid to mammalian cells?) | (Also lipofect wild-type plasmid to mammalian cells?) | ||
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| + | (Or: one day just dedicated to a journal article? Cf. F07Mod2) | ||
[[20.109(S08):Name| Module 2 Day 7: Name]]<br> | [[20.109(S08):Name| Module 2 Day 7: Name]]<br> | ||
Revision as of 03:59, 12 October 2007
Module 2 Day 1: Start-up protein engineering
(Initial reading about CaM-M13? Get calcium titration curve for WT plasmid? Need this day?)
(Students dissect pericam into constituent parts today or next time?)
Module 2 Day 2: Design protein modification
(Protein viewer, further reading about CaM-M13 to choose targets, plans primers for SDM)
Module 2 Day 3: Site-directed mutagenesis
(Staff or students do transformation/miniprep/transformation work? Timing issues, may take another lab day...)
Module 2 Day 4: Characterize protein expression
(IPTG induction, SDS-PAGE)
Module 2 Day 5: Protein binding assays
(Calcium titration curve, fluorescence-based)
Module 2 Day 6: Sequence analysis
(Also lipofect wild-type plasmid to mammalian cells?)
(Or: one day just dedicated to a journal article? Cf. F07Mod2)
(Microscopy assays with mammalian cells? MATLAB modeling?)
Module 2 Day 8: Student presentations