20.109(S07): Start-up expression engineering
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Contents
Introduction
SAGA intro 1. Gene expression in euk intimately related to chromatin dynamics 2. SAGA is 19 subunit complex req'd for chrom remodel and appropriate gene expression 3. Only 6 of SAGAs 19 subunits essential for viability. 4. Your mission, should you choose to accept it....
Protocols
Part 1: Choosing a SAGA subunit
Part 2: Designing deletion oligos
Part 3: PCR
- pRS406 template
DONE!