Difference between revisions of "20.109(F20): Fall 2020 schedule"

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| [https://be.mit.edu/directory/jacquin-c-niles JN]
 
| [https://be.mit.edu/directory/jacquin-c-niles JN]
 
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| [[20.109(F20):Homework#Due_M2D4|Homework due]]<br>[[20.109(F20): Mini-presentation| Mini-presentation due]] Sun, Oct 10 at 10 pm
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| [[20.109(F20):Homework#Due_M2D4|Homework due]]
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|--
 
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|  
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| <font color = 999999>T/W Oct 15/16</font color>
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| <font color = 999999>T Oct 13</font color>
 
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|  
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| <font color = 999999>Columbus day holiday</font color>
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| <font color = #0d368e>Columbus day holiday</font color>
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| [[20.109(F20): Data Summary| Data Summary revision due]] Wed, Oct 14 at 10 pm
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|--
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| M2D5
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| R Oct 15
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| [https://be.mit.edu/directory/jacquin-c-niles JN]
 
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|  
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| [[20.109(F20):Homework#Due_M2D5|Homework due]]<br>
 
|--
 
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| 2
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|  
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| 3
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| T Oct 22
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| R/F Oct 17/18
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|  
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D3 figures.pptx| Article Figures]]<br>[[Media:Fa19 M2D3 paper discussion slides.pdf| Lecture slides]]
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| [[20.109(F20):Journal club presentation| Journal club I]]
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| [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br> [[Media:M2D3_Fa18_LM_SP_edit.pdf | TR Prelab]]<br> [[Media:Fa19 M2D3 bcm marked.pdf | WF Prelab]]  
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|  
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| [[20.109(F19):Homework#Due_M2D3|Homework due]]<br>[[20.109(F19): Mini-presentation| Mini-presentation due]] Sat, Oct 19 at 10 pm
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|--
 
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| 2
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|  
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| 4
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| R Oct 24
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| T/W Oct 22/23
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D4 lecture slides.pdf|Lecture slides]]
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| [[20.109(F20):Journal club presentation| Journal club II]]
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| [[20.109(F19):Journal club presentation (Day4 and 6)| Journal club I]]
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|  
 
|  
 
|--
 
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| 2
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| M2D6
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| 5
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| T Oct 27
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| R/F Oct 24/25
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| [https://be.mit.edu/directory/jacquin-c-niles JN]
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D5 lecture slides v2.pdf| Lecture slides]]
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|  
−
| [[20.109(F19):Confirm gRNA sequence in pgRNA (Day5)| Confirm gRNA sequence in pgRNA]]<br> [[Media: M2D5_Fa19_LMmarked.pdf | TR Prelab]]<br> [[Media:Fa19 M2D5 cyk marked.pdf | WF Prelab]]
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| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D6|Homework due]]<br>
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| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D5|Homework due]]<br>[[20.109(F19): Data Summary| Data Summary revision due]] Sat, Oct 26 at 10 pm
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|--
 
|--
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| 2
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| M2D7
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| 6
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| R Oct 29
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| T/W Oct 29/30
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| [https://be.mit.edu/directory/jacquin-c-niles JN]
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D6 lecture slides.pdf|Lecture slides]]
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−
| [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]]
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|  
 
|  
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| [[20.109(F20):Homework#Due_M2D7|Homework due]]<br>
 
|--
 
|--
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| 2
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| colspan="6" |
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| 7
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−
| R/F Oct/Nov 31/1
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa19 M2D7 lecture slides.pdf| Lecture slides]]
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| [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br> [[Media: M2D7_Fa19_LM.pdf | TR Prelab]] <br> [[Media:Fa18 M2D7 bcm marked.pdf | WF Prelab]]
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−
| [[20.109(F19):Homework#Due_M2D7|Homework due]]<br>[https://be20109f19.blogspot.com/ Blog post due] Fri, Nov 1 at 10 pm
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|--
 
|--
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| 2
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| M3D1
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| 8
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| T Nov 3
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| T/W Nov 5/6
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
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| [[20.109(F19):Measure_fermentation_products_(Day8) | Measure fermentation products]]<br> [[Media: M2D8_Fa19_LMmarked.pdf | TR Prelab]] <br> [[Media:Fa19 M2D8 bcm marked.pdf | WF Prelab]]
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|
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| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D8|Homework due]]
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| [[20.109(F20):Homework#Due_M3D1|Homework due]]<br>[ Blog post due] Fri, Nov 1 at 10 pm
 
|--
 
|--
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| colspan="7" |
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| M3D2
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|--
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| R Nov 5
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| 3
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
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| 1
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|  
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| R/F Nov 7/8
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| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D2|Homework due]]
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| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L1 2019.pptx|Lecture slides]]
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| [[20.109(F19):Prepare for cellular thermal shift assay (Day1)| Prepare for cellular thermal shift assay]]<br> [[Media:Fa19 M3D1 prelab slides nllmarked.pdf| TR Prelab]] <br> [[Media:Fa19 M3D1 bcm marked.pdf | WF Prelab]]
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−
| [[20.109(F19):Homework#Due_M3D1|Homework due]] <br>
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|--
 
|--
 
|  
 
|  
 
|
 
|
−
| <font color = 999999>T/W Nov 12/13</font color>
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| <font color = 999999>T Nov 10</font color>
 
|  
 
|  
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| <font color = 999999>Veterans' day holiday</font color>
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| <font color = #0d368e>Veterans day holiday</font color>
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| [[20.109(F19): Research article| Research article due]]  Mon, Nov 11 at 10 pm <br>[https://be20109f19.blogspot.com/ Blog post due] Tue, Nov 12 at 10 pm
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| [[20.109(F20): Research article| Research article due]]  Wed, Nov 11 at 10 pm <br>[ Blog post due] Thu, Nov 12 at 10 pm
 
|--
 
|--
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| 3
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| M3D3
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| 2
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| R Nov 12
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| R/F Nov 14/15
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
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| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L2 2019.pptx|Lecture slides]]
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|  
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| [[20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)| Incubate with ligand and apply heat treatment for protein denaturation]]<br>[[Media:Fa19 M3D2 prelab slides nllmarked.pdf|TR Prelab]] <br> [[Media:191115 prelab CYK marked.pdf | WF Prelab]]
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| [[20.109(F20):Homework#Due_M3D3|Homework due]]<br>
−
| [[20.109(F19):Homework#Due_M3D2|Homework due]]<br>
+
 
|--  
 
|--  
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| 3
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|  
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| 3
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| T Nov 17
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| T/W Nov 19/20
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|  
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| [https://be.mit.edu/directory/angela-koehler AK]<br> [[Media:20109 Struntz Lecture 2019.pdf| Lecture slides]]
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| <font color = #0d368e>Research Proposal pitches</font color>
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| [[20.109(F19):Begin Western blot analysis (Day3)| Begin Western blot analysis]]<br>[[Media:Fa19 M3D3 prelab slides nllmarked.pdf|TR Prelab]] <br> [[Media:Fa19 M3D3 bcm.pdf | WF Prelab]]
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|
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D3| Homework due]]
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|--
 
|--
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| 3
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| M3D4
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| 4
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| R Nov 19
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| R/F Nov 21/22
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
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| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L4 2019.pdf| Lecture slides]]
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|  
−
| [[20.109(F19):Complete antibody staining for Western blot analysis (Day4)| Complete antibody staining for Western blot analysis]]<br> [[Media:M3D4 Fa19 bcm marked.pdf | TR Prelab]] <br> [[Media:M3D4WF Fa19 bcm marked.pdf  | WF Prelab]]
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| [[20.109(F20):Homework#Due_M3D4|Homework due]]
−
| [[20.109(F19):Homework#Due_M3D4|Homework due]]
+
 
|--
 
|--
 
|
 
|
−
|
+
| <font color = 999999>T Nov 24</font color>
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| T/W Nov 26/27
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|  
 
|  
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| <font color = #0101DF>'''Lecture: research proposal pitch, but no laboratory'''</font color>
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| <font color = #0d368e>Thanksgiving holiday</font color>
 
|  
 
|  
 
|--  
 
|--  
 
|
 
|
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| <font color = 999999>R Nov 26</font color>
 
|
 
|
−
| <font color = 999999>R/F Nov 28/29</font color>
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| <font color = #0d368e>Thanksgiving holiday</font color>
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|
+
−
| <font color = 999999>Thanksgiving holiday</font color>
+
 
|
 
|
 
|--
 
|--
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| 3
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| M3D5
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| 5
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| T Dec 1
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| T/W Dec 3/4 
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
−
| [https://be.mit.edu/directory/angela-koehler AK]<br>[[Media:20109 M3L5 2019.pdf| Lecture slides]]
+
|  
−
| [[20.109(F19):Data analysis and assignment preparation (Day5)| Data analysis and assignment preparation]]<br>[[Media:Fa19 M3D5 prelab slides nllmarked.pdf| TR Prelab]] <br> [[Media:Fa19 M3D5 bcm marked.pdf | WF Prelab]]
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| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D5|Homework due]]
−
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D5|Homework due]]
+
 
|--
 
|--
 
|  
 
|  
 +
| R Dec 3
 
|  
 
|  
−
| R/F Dec 5/6
+
| [[20.109(F20):Research proposal presentation| Research proposal presentations]]
−
|
+
| [ Blog post due] Fri, Dec 4 at 10 pm
−
| [[20.109(F19):Research proposal presentation| Research proposal presentations]]
+
−
| [https://be20109f19.blogspot.com/ Blog post due] Fri, Dec 6 at 10 pm
+
 
|--
 
|--
−
| colspan="7" |
+
| colspan="6" |
 
|--
 
|--
 
|  
 
|  
 +
| T Dec 8
 
|  
 
|  
−
| T Dec 10
+
| <font color =  #0d368e>'''Feedback session'''</font color>
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|
+
| [[20.109(F20): Mini-report | Mini-report due]] Mon, Dec 7 at 10 pm <br>
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| <font color =  #0101DF>'''Feedback and celebratory lunch'''</font color>
+
−
| [[20.109(F19): Mini-report | Mini-report due]] Mon, Dec 9 at 10 pm <br>
+
 
|}
 
|}
 
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Revision as of 19:40, 10 July 2020

20.109(F20): Laboratory Fundamentals of Biological Engineering

Fa20 banner image v2.png

Fall 2020 schedule        FYI        Assignments        Homework        Communication |        Accessibility

       M1: Genomic instability        M2: Drug discovery        M3: Metabolic engineering       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T Sept 1 NLL
 Orientation and laboratory tour
M1D1 R Sep 3 BE Laboratory orientation quiz
Homework due
M1D2 T Sep 8 BE Homework due
M1D3 R Sep 10 BE Homework due
M1D4 T Sep 15 BE Homework due
M1D5 R Sep 17 BE Laboratory quiz
Homework due
M1D6 T Sep 22 BE Homework due
M1D7 R Sep 24 BE Homework due
M2D1 T Sep 29 JN Laboratory quiz
Homework due
M2D2 R Oct 1 JN Homework due
Data Summary draft due Sun, Oct 3 at 10 pm
[ Blog post due] Mon, Oct 4 at 10 pm
M2D3 T Oct 6 JN Homework due
M2D4 R Oct 8 JN Homework due
Mini-presentation due Sun, Oct 10 at 10 pm
T Oct 13 Columbus day holiday Data Summary revision due Wed, Oct 14 at 10 pm
M2D5 R Oct 15 JN Homework due
T Oct 22 Journal club I
R Oct 24 Journal club II
M2D6 T Oct 27 JN Laboratory quiz
Homework due
M2D7 R Oct 29 JN Homework due
M3D1 T Nov 3 NLL
 Homework due
[ Blog post due] Fri, Nov 1 at 10 pm
M3D2 R Nov 5 NLL
 Laboratory quiz
Homework due
T Nov 10 Veterans day holiday Research article due Wed, Nov 11 at 10 pm
[ Blog post due] Thu, Nov 12 at 10 pm
M3D3 R Nov 12 NLL
 Homework due
T Nov 17 Research Proposal pitches
M3D4 R Nov 19 NLL
 Homework due
T Nov 24 Thanksgiving holiday
R Nov 26 Thanksgiving holiday
M3D5 T Dec 1 NLL
 Laboratory quiz
Homework due
R Dec 3 Research proposal presentations [ Blog post due] Fri, Dec 4 at 10 pm
T Dec 8 Feedback session Mini-report due Mon, Dec 7 at 10 pm
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