20.109(F18): Fall 2018 schedule

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20.109(F18): Laboratory Fundamentals of Biological Engineering

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Fall 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Engineering biomaterials              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (16-220)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sept 6/7 NLL
Orientation lecture
Orientation
Prelab T/R
Prelab W/F
1 1 T/W Sept 11/12 BE Practice tissue culture and prepare microwell array
Prelab T/R
Prelab W/F
Laboratory orientation quiz
Homework due
1 2 R/F Sept 13/14 BE Design cell loading optimization experiment and research cell lines
Prelab T/R
Prelab W/F
Homework due
1 3 T/W Sept 18/19 BE Prepare and treat cells for genomic instability experiment
Prelab T/R
Prelab W/F
Homework due
R/F Sept 20/21 BE Lecture, but no laboratory
Career fair student holiday
1 4 T/W Sept 25/26 Comm Lab Complete genomic instability experiment and load cells for sub-nuclear foci assay
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
1 5 R/F Sept 27/28 BE Analyze instability experiment data and treat cells for sub-nuclear foci assay
Prelab T/R
Prelab W/F
Homework due
1 6 T/W Oct 2/3 BE Complete sub-nuclear foci assay
Prelab T/R
Prelab W/F
Homework due
1 7 R/F Oct 4/5 BE Practice statistical analysis methods and complete data analysis
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
T/W Oct 9/10 Columbus day holiday Data Summary draft due Wed, Oct 10 at 10 pm
Blog post due Thurs, Oct 11 at 10 pm
2 1 R/F Oct 11/12 NLL
M2D1 lecture
Complete in silico cloning
Prelab T/R
Prelab W/F
Homework due
Mini-presentation due Sat, Oct 13 at 10 pm
2 2 T/W Oct 16/17 NLL
M2D2 lecture
Prelab T/R
Design gRNA for CRISPRi
Homework due
2 3 R/F Oct 18/19 NLL
Generate gRNA plasmid
Homework due
Data Summary revision due Sat, Oct 20 at 10 pm
2 4 T/W Oct 23/24 NLL
Journal club I
2 5 R/F Oct 25/26 NLL
Confirm gRNA sequence
Laboratory quiz
Homework due
2 6 T/W Oct 30/31 NLL
Journal club II
2 7 R/F Nov 1/2 NLL
Induce CRISPRi system
Homework due
Blog post due Fri, Nov 2 at 10 pm
2 8 T/W Nov 6/7 NLL
Measure fermentation products
Laboratory quiz
Homework due
3 1 R/F Nov 8/9 AB Grow phage-based active (cathode) material
Homework due
T/W Nov 13/14 Veterans' day holiday Research article due Mon, Nov 12 at 10 pm
Blog post due Tue, Nov 13 at 10 pm
3 2 R/F Nov 15/16 AB Purify phage-based active (cathode) material
Homework due
T/W Nov 20/21 AB
Lecture, but no laboratory
R/F Nov 22/23 Thanksgiving holiday
3 3 T/W Nov 27/28 AB Construct cathode with phage-based active material
Laboratory quiz
Homework due
3 4 R/F Nov/Dec 29/30 AB
Visualize phage nanowires using transmission electron microscopy
Homework due
3 5 T/W Dec 4/5 AB
Assemble and test batteries
Laboratory quiz
Homework due
R/F Dec 6/7 Research proposal presentations Research proposal presentation due Thu/Fri, Dec 6/7 at 1 pm
Blog post due Sat, Dec 8 at 10 pm
T Dec 11 Feedback and celebratory lunch Mini-report due Mon, Dec 10 at 10 pm