Difference between revisions of "20.109(F18):Class data"

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(→‎Fermentation product and gene targeted:)
Line 123: Line 123:
 
|'''Gene targeted by CRISPRi gRNA'''
 
|'''Gene targeted by CRISPRi gRNA'''
 
|'''gRNA (DNA) sequence (without tag at 3' end)'''
 
|'''gRNA (DNA) sequence (without tag at 3' end)'''
 +
|'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)'''
 +
|'''Target template or nontemplate strand'''
 
|-
 
|-
 
|TR red
 
|TR red
Line 128: Line 130:
 
| pta
 
| pta
 
| TGCGCCCGATCAGACTACGACTATC
 
| TGCGCCCGATCAGACTACGACTATC
 +
|
 +
|
 
|-
 
|-
 
|TR orange
 
|TR orange
Line 133: Line 137:
 
|ldhA
 
|ldhA
 
|gttgcaggtacttcttgtcgt
 
|gttgcaggtacttcttgtcgt
 +
|
 +
|
 
|-
 
|-
 
|TR green
 
|TR green
Line 138: Line 144:
 
|adhE
 
|adhE
 
|TACTAAAAAAGTTTAACATTATCA
 
|TACTAAAAAAGTTTAACATTATCA
 +
|
 +
|
 
|-
 
|-
 
|TR pink
 
|TR pink
Line 143: Line 151:
 
|Citrate Synthase (gltA)
 
|Citrate Synthase (gltA)
 
|tgagttttgcttttgtatcagccat
 
|tgagttttgcttttgtatcagccat
 +
|
 +
|
 
|-
 
|-
 
|TR purple
 
|TR purple
Line 148: Line 158:
 
|ldhA
 
|ldhA
 
|TAGTAGCTTAAATGTGATTCAACAT
 
|TAGTAGCTTAAATGTGATTCAACAT
 +
|
 +
|
 
|}
 
|}
  
Line 157: Line 169:
 
|'''Gene targeted by CRISPRi gRNA'''
 
|'''Gene targeted by CRISPRi gRNA'''
 
|'''gRNA (DNA) sequence (without tag at 3' end)'''
 
|'''gRNA (DNA) sequence (without tag at 3' end)'''
 +
|'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)'''
 +
|'''Target template or nontemplate strand'''
 
|-
 
|-
 
|WF yellow
 
|WF yellow
Line 162: Line 176:
 
| adhE
 
| adhE
 
| ATTCGAGCAGATGATTTACTAAAAA
 
| ATTCGAGCAGATGATTTACTAAAAA
 +
|
 +
|
 
|-
 
|-
 
|WF green
 
|WF green
Line 167: Line 183:
 
| adhE
 
| adhE
 
| TTACTAAAAAAGTTTAACATTATCA
 
| TTACTAAAAAAGTTTAACATTATCA
 +
|
 +
|
 
|-
 
|-
 
|WF blue
 
|WF blue
Line 172: Line 190:
 
| adhE
 
| adhE
 
| TTCGAGCAGATGATTTACTAAA
 
| TTCGAGCAGATGATTTACTAAA
 +
|
 +
|
 
|}
 
|}

Revision as of 21:08, 30 October 2018

20.109(F18): Laboratory Fundamentals of Biological Engineering

Fa18 20109 banner image.png

Fall 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Engineering biomaterials              

Module 1

Cell Loading Data

TR and WF cell loading data

CometChip Data

Team Analyzed Data
T/R red Analyzed CometChip data
T/R orange Analyzed CometChip data
T/R green Analyzed CometChip data
T/R pink Analyzed CometChip data
T/R purple Analyzed CometChip data


Team Analyzed Data
W/F yellow Analyzed CometChip data
W/F green Analyzed CometChip data
W/F blue Analyzed CometChip data
W/F pink Analyzed CometChip data


gamma-H2AX Data

Team Recovery time (min) Raw gamma-H2AX Data Analyzed gamma-H2AX Data
T/R red 30 raw data analyzed data
T/R orange 30 raw data analyzed data
T/R green 30 raw data analyzed data
T/R pink 30 raw data analyzed data
T/R purple 60 raw data analyzed data


Team Recovery time (min) Raw gamma-H2AX Data Analyzed gamma-H2AX Data
W/F yellow 30 raw data analyzed data
W/F green 30 raw data analyzed data
W/F blue 60 raw data analyzed data
W/F pink 30 raw data analyzed data

Module 2

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
TR red E pta TGCGCCCGATCAGACTACGACTATC
TR orange E ldhA gttgcaggtacttcttgtcgt
TR green A adhE TACTAAAAAAGTTTAACATTATCA
TR pink A Citrate Synthase (gltA) tgagttttgcttttgtatcagccat
TR purple A ldhA TAGTAGCTTAAATGTGATTCAACAT

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
WF yellow A adhE ATTCGAGCAGATGATTTACTAAAAA
WF green Acetate adhE TTACTAAAAAAGTTTAACATTATCA
WF blue A adhE TTCGAGCAGATGATTTACTAAA
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