Difference between revisions of "20.109(F18):Class data"

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(→‎W/F)
(→‎Fermentation product and gene targeted:)
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|'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)'''
 
|'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)'''
 
|'''Target template or nontemplate strand'''
 
|'''Target template or nontemplate strand'''
 +
|'''Colorimetric Assay Results'''
 
|-
 
|-
 
|TR red
 
|TR red
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| Middle of the operon
 
| Middle of the operon
 
| Non-template
 
| Non-template
 +
|[[File: TRRed_ethanol_rawdata.xlsx | Raw data ]]
 
|-
 
|-
 
|TR orange
 
|TR orange
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|32 bps downstream from start of gene
 
|32 bps downstream from start of gene
 
|Template Strand
 
|Template Strand
 +
|[[File: TROrange_ethanol_rawdata.xlsx | Raw data ]]
 
|-
 
|-
 
|TR green
 
|TR green
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|locus targeted: -50 upstream (promoter)
 
|locus targeted: -50 upstream (promoter)
 
|Template strand
 
|Template strand
 +
|[[File: TRGreen_acetate_rawdata.xlsx | Raw data ]]
 
|-
 
|-
 
|TR pink
 
|TR pink
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|Beginning of gene
 
|Beginning of gene
 
|Non-template Strand
 
|Non-template Strand
 +
|[[File: TRPink_acetate_rawdata.xlsx | Raw data ]]
 
|-
 
|-
 
|TR purple
 
|TR purple
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|Locus targeted: -40 upstream region (promoter)
 
|Locus targeted: -40 upstream region (promoter)
 
|Non-template strand
 
|Non-template strand
 +
|[[File: TRPurple_acetate_rawdata.xlsx | Raw data ]]
 
|
 
|
 
|}
 
|}

Revision as of 16:33, 6 November 2018

20.109(F18): Laboratory Fundamentals of Biological Engineering

Fa18 20109 banner image.png

Fall 2018 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Engineering biomaterials              

Module 1

Cell Loading Data

TR and WF cell loading data

CometChip Data

Team Analyzed Data
T/R red Analyzed CometChip data
T/R orange Analyzed CometChip data
T/R green Analyzed CometChip data
T/R pink Analyzed CometChip data
T/R purple Analyzed CometChip data


Team Analyzed Data
W/F yellow Analyzed CometChip data
W/F green Analyzed CometChip data
W/F blue Analyzed CometChip data
W/F pink Analyzed CometChip data


gamma-H2AX Data

Team Recovery time (min) Raw gamma-H2AX Data Analyzed gamma-H2AX Data
T/R red 30 raw data analyzed data
T/R orange 30 raw data analyzed data
T/R green 30 raw data analyzed data
T/R pink 30 raw data analyzed data
T/R purple 60 raw data analyzed data


Team Recovery time (min) Raw gamma-H2AX Data Analyzed gamma-H2AX Data
W/F yellow 30 raw data analyzed data
W/F green 30 raw data analyzed data
W/F blue 60 raw data analyzed data
W/F pink 30 raw data analyzed data

Module 2

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand Colorimetric Assay Results
TR red E pta TGCGCCCGATCAGACTACGACTATC Middle of the operon Non-template File:TRRed ethanol rawdata.xlsx
TR orange E ldhA gttgcaggtacttcttgtcgt 32 bps downstream from start of gene Template Strand File:TROrange ethanol rawdata.xlsx
TR green A adhE TACTAAAAAAGTTTAACATTATCA locus targeted: -50 upstream (promoter) Template strand File:TRGreen acetate rawdata.xlsx
TR pink A Citrate Synthase (gltA) tgagttttgcttttgtatcagccat Beginning of gene Non-template Strand File:TRPink acetate rawdata.xlsx
TR purple A ldhA TAGTAGCTTAAATGTGATTCAACAT Locus targeted: -40 upstream region (promoter) Non-template strand File:TRPurple acetate rawdata.xlsx

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
WF yellow A adhE ATTCGAGCAGATGATTTACTAAAAA
WF green Acetate adhE TTACTAAAAAAGTTTAACATTATCA
WF blue A adhE TTCGAGCAGATGATTTACTAAA locus targeted: -65 upstream (promoter) Template Strand

gRNA_target Sequencing Results

T/R

Team pgRNA sequencing
red files
orange files
green files
pink files
purple files

W/F

Team pgRNA sequencing
yellow files
green files
blue files
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