Difference between revisions of "20.109(F17): Notes for M1"
From Course Wiki
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*100ml glass bottles near weigh station | *100ml glass bottles near weigh station | ||
*pre-cut gel bond at front bench | *pre-cut gel bond at front bench | ||
| + | *Secure line II pen | ||
===M1D2=== | ===M1D2=== | ||
Revision as of 17:45, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of DMEM media
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
- Secure line II pen
M1D2
Per group, aliquot
- in main lab
- 1x PBS glass bottle
- 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
- print a large scheme of the plate so they can keep track of how to load chips
- and separately, in the TC hood,
- 5 mL 1x PBS
- 10 mL MEF media
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- team sticker to identify glass slides
- printout of today's protocol
- large scheme of the plate to hang in TC so they can keep track of how to load chips
