Difference between revisions of "20.109(F16): TA notes for M2"
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MAXINE JONAS (Talk | contribs) (Created page with "{{Template:20.109(F16)}} <div style="padding: 10px; width: 790px; border: 5px solid #000FFF;"> ===M2D1=== Per group, aliquot *30 μL nuclease-free water *5 μL pdCas9 (a...") |
MAXINE JONAS (Talk | contribs) (→M2D1) |
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Per group, aliquot | Per group, aliquot | ||
*30 μL nuclease-free water | *30 μL nuclease-free water | ||
| − | *5 μL pdCas9 (at | + | *5 μL pdCas9 (ideally at 500 ng/μL) |
On front bench, have | On front bench, have | ||
Revision as of 19:36, 19 October 2016
M2D1
Per group, aliquot
- 30 μL nuclease-free water
- 5 μL pdCas9 (ideally at 500 ng/μL)
On front bench, have
- restriction enzymes in -20 °C block
- NEB buffers
- list of 20.109-owned restriction enzymes
Fa16: all done by 5pm
M2D2
Per group,
- pour 1/2 agarose gel (only 5 lanes needed)
- thaw 4 samples of confirmation digests
- aliquot 10 μL of 6X loading buffer
- thaw 20 μL of 1 kb ladder
Call IDT to request expedited FedEx shipping of gRNA oligos.
Fa16: all done by 4:30pm
Should schedule BE Comm Lab workshop on Journal Club on this day.