20.109(F16):Asses DNA repair variability with comet chip (Day4)

Introduction

Today you will load cells into the comet chip you prepared during the previous laboratory class. Cell loading refers to the process by which the cells are added to the comet chip. Briefly, you will add media containing cells over the agarose-based comet chip and gravity will pull the cells down and 'load' them into the comet chip. The details of this process will be the basis of the experiments you design and perform during this laboratory meeting.

Experimental design is

Protocols

Part 1: Load cells and induce DNA damage

Part 2: Use comet chip to assess repair capacity

Reagents

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