Difference between revisions of "20.109(F15): TA notes for module 1"
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[[20.109(F09)| 20.109(F09)]](archive) <br> | [[20.109(F09)| 20.109(F09)]](archive) <br> | ||
[[20.109(F08)| 20.109(F08)]] (archive)<br> | [[20.109(F08)| 20.109(F08)]] (archive)<br> | ||
− | [ | + | [http://openwetware.org/wiki/BE.109:DNA_engineering | BE.109(S06)] (archive)<br> |
[[20.109(S08):Module 1| 20.109(S08)]] (archive)<br> | [[20.109(S08):Module 1| 20.109(S08)]] (archive)<br> | ||
[[Media:TA NotebookGrading.jpg| Notebook grading checklist]] | [[Media:TA NotebookGrading.jpg| Notebook grading checklist]] | ||
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# Buy fresh ligation buffer, T4 ligase from NEB | # Buy fresh ligation buffer, T4 ligase from NEB | ||
# 3 M sodium acetate (one 100 μL aliquot/group) | # 3 M sodium acetate (one 100 μL aliquot/group) | ||
− | # Yeast tRNA (one 25 μL aliquot/group) | + | # Yeast tRNA 12.5mg/ml (one 25 μL aliquot/group) |
# Cold 100% ethanol (one 1 mL aliquot/group) | # Cold 100% ethanol (one 1 mL aliquot/group) | ||
# Cold 70% ethanol (one 3 mL aliquots/group) | # Cold 70% ethanol (one 3 mL aliquots/group) | ||
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===Agarose gel=== | ===Agarose gel=== | ||
− | # DNA gel: 1% agarose gel in 1X TAE, 1 g agarose, 100mL 1X TAE, 10 | + | # DNA gel: 1% agarose gel in 1X TAE, 1 g agarose, 100mL 1X TAE, 10 |