Difference between revisions of "20.109(F15): TA's notes for module 3"
From Course Wiki
MAXINE JONAS (Talk | contribs) (→Day 1) |
MAXINE JONAS (Talk | contribs) (→Day 1) |
||
| Line 30: | Line 30: | ||
'''In 4 °C fridge:''' | '''In 4 °C fridge:''' | ||
*orbital shaker (speed 120) | *orbital shaker (speed 120) | ||
| + | |||
| + | ===[[20.109(F15):Purify active materials (Day2)| Day 2]]=== | ||
| + | |||
| + | '''Between Day 1 and Day 2:''' | ||
| + | |||
| + | '''On the instructors' bench:''' | ||
| + | *50 mL conical tubes | ||
| + | *15 mL conical tubes | ||
| + | *tweezers | ||
| + | *TEM grids for practice | ||
| + | *sign-up sheet for location of samples in TEM holder | ||
| + | |||
| + | '''On students' benches:''' | ||
| + | *Na:PO<sub>4</sub> | ||
Revision as of 21:02, 17 November 2015
Day 1
The week preceding the lab:
- Make LB:
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- 1 L deionized water
- autoclave 30 minutes with stirbar.
On the instructors' bench:
- 50 mL conical tubes
- 25 mL pipets
- a couple of motorized pipet aids
- 2 mL eppendorf tubes
- TBS: 500 mL
- PEG/NaCl: 500 mL
- deionized water: 50 mL
- quartz cuvettes
On the students' bench:"
- bucket of ice
- 2 x 40 mL overnight culture of XL1-Blue infected with M13 phage
- 250 mL erlenmeyer flask
By the scale:
- (NH4)2Fe(SO4)2
- stirbars
In 4 °C fridge:
- orbital shaker (speed 120)
Day 2
Between Day 1 and Day 2:
On the instructors' bench:
- 50 mL conical tubes
- 15 mL conical tubes
- tweezers
- TEM grids for practice
- sign-up sheet for location of samples in TEM holder
On students' benches:
- Na:PO4
