Difference between revisions of "20.109(F15): TA's notes for module 3"
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MAXINE JONAS (Talk | contribs) (→Day 1) |
MAXINE JONAS (Talk | contribs) (→Day 1) |
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| Line 12: | Line 12: | ||
*50 mL conical tubes | *50 mL conical tubes | ||
*25 mL pipets | *25 mL pipets | ||
| + | *a couple of motorized pipet aids | ||
*2 mL eppendorf tubes | *2 mL eppendorf tubes | ||
*TBS: 500 mL | *TBS: 500 mL | ||
*PEG/NaCl: 500 mL | *PEG/NaCl: 500 mL | ||
| + | *deionized water: 50 mL | ||
*quartz cuvettes | *quartz cuvettes | ||
Revision as of 22:27, 12 November 2015
Day 1
The week preceding the lab:
- Make LB:
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- 1 L deionized water
- autoclave 30 minutes with stirbar.
On the instructors' bench:
- 50 mL conical tubes
- 25 mL pipets
- a couple of motorized pipet aids
- 2 mL eppendorf tubes
- TBS: 500 mL
- PEG/NaCl: 500 mL
- deionized water: 50 mL
- quartz cuvettes
On the students' bench:"
- bucket of ice
- 2 x 40 mL overnight culture of XL1-Blue infected with M13 phage
- 250 mL erlenmeyer flask
By the scale:
- (NH4)2Fe(SO4)2
- stirbars
In 4 °C fridge:
- orbital shaker (speed 120)
