Difference between revisions of "20.109(F15): TA's notes for module 3"
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MAXINE JONAS (Talk | contribs) (→Day 1) |
MAXINE JONAS (Talk | contribs) (→Day 1) |
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*PEG/NaCl: 500 mL | *PEG/NaCl: 500 mL | ||
*quartz cuvettes | *quartz cuvettes | ||
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+ | '''On the students' bench:" | ||
+ | *bucket of ice | ||
+ | *2 x 40 mL overnight culture of XL1-Blue infected with M13 phage |
Revision as of 22:17, 12 November 2015
Day 1
The week preceding the lab:
- Make LB:
- 10 g tryptone
- 5 g yeast extract
- 10 g NaCl
- 1 L deionized water
- autoclave 30 minutes with stirbar.
On the instructors' bench:
- 50 mL conical tubes
- 25 mL pipets
- 2 mL eppendorf tubes
- TBS: 500 mL
- PEG/NaCl: 500 mL
- quartz cuvettes
On the students' bench:"
- bucket of ice
- 2 x 40 mL overnight culture of XL1-Blue infected with M13 phage