Difference between revisions of "20.109(F09): Mod 3 Day 2 Phage nanowires"
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==Protocols== | ==Protocols== | ||
===Part 1: Dilute the 8#9 phage stock=== | ===Part 1: Dilute the 8#9 phage stock=== | ||
− | Count the number of plaques on your phage titering plates from last time. Calculate the concentration of phage in your undiluted purified phage sample. Be sure to take into account each dilution when you try this calculation. Express the concentration as PFU/ul and determine the volume of phage needed to make 10 ml of 3.5x10^7 PFU/ | + | Count the number of plaques on your phage titering plates from last time. Calculate the concentration of phage in your undiluted purified phage sample. Be sure to take into account each dilution when you try this calculation. Express the concentration as PFU/ul and determine the volume of phage needed to make 10 ml of 3.5x10^7 PFU/ |