Difference between revisions of "20.109(F07): M13.1 and MDS titers"

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(→‎Blue Team)
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*Description of M13.1 phage:  
 
*Description of M13.1 phage:  
 
*Description of MDS strain:  
 
*Description of MDS strain:  
−
*Any notes about titers presented here:
+
*Any notes about titers presented here:M13K07 control not grown in parallel
 +
====Plaque numbers====
 +
<center>
 +
{| border="1"
 +
|
 +
|phage = M13K07, strain = MG1655 
 +
|phage = M13K07, strain = MDS
 +
|phage = M13.1, strain = MG1655
 +
|phage = M13.1, strain = MDS
 +
|-
 +
| 10-6
 +
|~1000
 +
| ND
 +
|~1000
 +
|~1500
 +
|-
 +
| 10-8
 +
|16
 +
|ND
 +
|10
 +
|42
 +
|-
 +
| 10-10
 +
|
 +
|
 +
|
 +
|
 +
|}
 +
</center>
 +
====Titer====
 +
<center>
 +
{| border="1"
 +
|
 +
|phage = M13K07, strain = MG1655 
 +
|phage = M13K07, strain = MDS
 +
|phage = M13.1, strain = MG1655
 +
|phage = M13.1, strain = MDS
 +
|-
 +
| titer (PFU/ml)
 +
|
 +
|
 +
|
 +
|
 +
|}
 +
</center>
 +
 
 
===Yellow Team===
 
===Yellow Team===
 
*Team members:
 
*Team members:

Revision as of 14:44, 5 December 2007


20.109(F07): Laboratory Fundamentals of Biological Engineering

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All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

T/R lab

Green Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Purple Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Red Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Pink Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Blue Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:M13K07 control not grown in parallel

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~1000 ND ~1000 ~1500
10-8 16 ND 10 42
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Yellow Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

W/F lab

Green Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Purple Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Red Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Pink Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Blue Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Yellow Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:
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