Difference between revisions of "20.109(F07): Journal article discussion"

Revision as of 02:40, 1 September 2007

20.109(F07): Laboratory Fundamentals of Biological Engineering

Home People Schedule Fall 2007 Assignments Lab Basics OWW Basics
Genome Engineering Expression Engineering Biomaterials Engineering

Calculate the volume for 4 ug, 2 ug, 1 ug and 0.5 ug of each RNA sample that you prepared. Show your work. Save a copy of your answer since you'll need to know this volume to perform your experiment.
Please familiarize yourself with the basics of microarrays by reading NCBI's primer on the technique.

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+==Journal articles to be discussed==
-Krueger U, Bergauer T, Kaufmann B, Wolter I, Pilk S, Heider-Fabian M, Kirch S, Artz-Oppitz C, Isselhorst M, Konrad J.Insights into Effective RNAi Gained from Large-Scale siRNA Validation Screening. Oligonucleotides. 2007 Summer;17(2):237-50.
+==Questions to guide your reading==
-<br>
+==For next time==
+# Calculate the volume for 4 ug, 2 ug, 1 ug and 0.5 ug of each RNA sample that you prepared. Show your work. Save a copy of your answer since you'll need to know this volume to perform your experiment.
-Svoboda P.Curr Opin Mol Ther. 2007 Jun;9(3):248-57. Off-targeting and other non-specific effects of RNAi experiments in mammalian cells.
+# Please familiarize yourself with the basics of microarrays by reading [http://www.ncbi.nih.gov/About/primer/microarrays.html NCBI's primer on the technique].
-svobodap@img.cas.cz
-<br>
-Tschaharganeh D, Ehemann V, Nussbaum T, Schirmacher P, Breuhahn K. Non-specific Effects of siRNAs on Tumor Cells with Implications on Therapeutic Applicability Using RNA Interference. Pathol Oncol Res. 2007;13(2):84-90. Epub 2007 Jul 3.