20.109(F07): ECD assembly

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20.109(F07): Laboratory Fundamentals of Biological Engineering

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Introduction

Protocol

Part 1: Phage titers for M13.1 and MDS

Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the data collection page. Since we might want to publish this class data in a peer reviewed journal, it will be particularly important for you to provide complete and accurate information as well as for you to "sign" your work so we can attribute you properly.

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Part 2: Li overlay

DONE!

For next time

  1. titer?, summary paragraph
  2. summary of ECD, variables and effects
  3. final presentation directions

Reagents

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