Difference between revisions of "20.109(F07): ECD assembly"
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==Protocol== | ==Protocol== | ||
===Part 1: Phage titers for M13.1 and MDS=== | ===Part 1: Phage titers for M13.1 and MDS=== | ||
− | Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the [[20.109(F07): M13.1 and MDS titers| data collection page]]. | + | Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the [[20.109(F07): M13.1 and MDS titers| data collection page]]. Since we might want to publish this class data in a peer reviewed journal, it will be particularly important for you to provide complete and accurate information as well as for you to "sign" your work so we can attribute you properly. |
====Plaque numbers==== | ====Plaque numbers==== | ||
<center> | <center> |
Revision as of 18:30, 2 November 2007
Contents
Introduction
Protocol
Part 1: Phage titers for M13.1 and MDS
Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the data collection page. Since we might want to publish this class data in a peer reviewed journal, it will be particularly important for you to provide complete and accurate information as well as for you to "sign" your work so we can attribute you properly.
Plaque numbers
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
10-6 | ||||
10-8 | ||||
10-10 |
Titer
phage = M13K07, strain = MG1655 | phage = M13K07, strain = MDS | phage = M13.1, strain = MG1655 | phage = M13.1, strain = MDS | |
titer (PFU/ml) |
Part 2: Li overlay
DONE!
For next time
- titer?, summary paragraph
- summary of ECD, variables and effects
- final presentation directions