Difference between revisions of "20.109(F07): ECD assembly"

From Course Wiki
Jump to: navigation, search
Line 2: Line 2:
 
==Introduction==
 
==Introduction==
 
==Protocol==
 
==Protocol==
−
===Part 1: Plaques===
+
===Part 1: Phage titers for M13.1 and MDS===
 +
Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the [[20.109(F07): M13.1 and MDS titers| data collection page]].
 +
====Plaque numbers====
 +
<center>
 +
{| border="1"
 +
|
 +
|phage = M13K07, strain = MG1655 
 +
|phage = M13K07, strain = MDS
 +
|phage = M13.1, strain = MG1655
 +
|phage = M13.1, strain = MDS
 +
|-
 +
| 10-6
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| 10-8
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| 10-10
 +
|
 +
|
 +
|
 +
|
 +
|}
 +
</center>
 +
====Titer====
 +
<center>
 +
{| border="1"
 +
|
 +
|phage = M13K07, strain = MG1655 
 +
|phage = M13K07, strain = MDS
 +
|phage = M13.1, strain = MG1655
 +
|phage = M13.1, strain = MDS
 +
|-
 +
| titer (PFU/ml)
 +
|
 +
|
 +
|
 +
|
 +
|}
 +
</center>
 
===Part 2: Li overlay===
 
===Part 2: Li overlay===
 
DONE!
 
DONE!

Revision as of 18:25, 2 November 2007


20.109(F07): Laboratory Fundamentals of Biological Engineering

Macintosh HD-Users-nkuldell-Desktop-bldg10crop.png

Home        People        Schedule Fall 2007        Assignments        Lab Basics        OWW Basics       
Genome Engineering        Expression Engineering        Biomaterials Engineering              

Introduction

Protocol

Part 1: Phage titers for M13.1 and MDS

Record the number of plaques for each plate you prepared last time, and then use the most "countable" to calculate PFU/ml for the M13K07/M13.1 and WT/MDS pairs. To collect the class's data in one place, you should then copy two tables to the data collection page.

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Part 2: Li overlay

DONE!

For next time

  1. titer?, summary paragraph
  2. summary of ECD, variables and effects
  3. final presentation directions

Reagents

Retrieved from "http://engineerbiology.org/w/index.php?title=20.109(F07):_ECD_assembly&oldid=13714"