Difference between revisions of "20.109(F07): DNA ligation and bacterial transformation"
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Hopefully, your ligation reactions will generate your desired construct, namely the M13K07 backbone carrying a short added sequence in the gene for p3. Alternative ligation products may arise, including a simple reclosing of the singly cut M13K07 backbone. We will remove these easy to form but undesired products with a trick called a "kill cut," described below. | Hopefully, your ligation reactions will generate your desired construct, namely the M13K07 backbone carrying a short added sequence in the gene for p3. Alternative ligation products may arise, including a simple reclosing of the singly cut M13K07 backbone. We will remove these easy to form but undesired products with a trick called a "kill cut," described below. | ||
− | You will transform today's ligation reactions into bacteria. During | + | You will transform today's ligation reactions into bacteria. During |