Thermal Quenching Model
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Revision as of 22:23, 22 July 2011 by Kelly Drinkwater (Talk | contribs)
Zipper et al says linear.
The Roche LightCycler paper [1] also shows a linear dependence, or at least it looks pretty linear (Fig. 2). Interesting -- they have a melting temp of about 85 degrees, with just normal DNA of 536bp length. We should try that. Anyway, my suspicion is that "linear" is a fine model of thermal quenching, and we should de-prioritize thermal quenching investigations.
Polymers & stuff
- Polyvinyl alcohol
- Polyvinylpyrrolidone
- Polyethylene glycol (?)
- Polysorbate (?)
- Polyvinyl acetate (?)
- Matrigel
- Mineral oil
- Glycerol
- Gelatin
- Agar(ose)