20.109(F08): Protein engineering research article

Primer design in Materials and Methods
Figures to include:

• Agarose Gel showing transformed frag from PCR/Qkit
• Transformation results
• Western showing TAP
• Growth Phenotypes
• Array data

• Figure N: Growth phenotypes. If you'd like an example of a spot test figure from a published article, consider modeling your figure and legend along the lines of Figure 2 from Monehan et al "Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast"Nature Structural & Molecular Biology (2008) 15, 873 - 880 here
• Figure N: Western blot for TAP tag. If you'd like an example of a figure that considers protein levels, consider modeling your figure and legend along the lines of figure 6 from Laprade et al. "Characterization of New Spt3 and TATA-Binding Protein Mutants of Saccharomyces cerevisiae: Spt3–TBP Allele-Specific Interactions and Bypass of Spt8" Genetics(2007)177: 2007-2017 here