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• 20.109(S14):DNA cloning (Day4)
  ... realize that even with high efficiency cells only 1 DNA molecule in about 10,000 is successfully transformed. | [http://www.neb.com/nebecomm/products/productN3231.asp DNA ladder] (load 10 μL)
  15 KB (2,521 words) - 13:58, 29 July 2015
• 20.109(S14):Initiate cell culture (Day2)
  ... rest of the afternoon however you see fit. (Whether that involves the FNT assignment, notebook prep, or a walk in the sunshine - are we still expecting sunshine ...ot shared, one per person), and the large are for temporary waste in steps 10-12 below (shared, one per hood).
  9 KB (1,550 words) - 13:59, 29 July 2015
• 20.109(S14):Lab tour
  ...ay. Afterward, you may have some time remaining to begin the For Next Time assignment, and are strongly encouraged to do so. We'll re-group at 4:15 pm for a BioS #Using your P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuve
  28 KB (4,689 words) - 13:59, 29 July 2015
• 20.109(S14):Begin Western protein analysis (Day2)
  ...ence of specific antibodies. Even the very best antisera have no more than 10% of their antibodies directed against a particular antigen. The quality of ...apacity for each lane is usually 100 to 200 ug (that would be 20 ul of a 5-10 ug/ul protein preparation). Thus 1 ng represents a protein that is approxim
  16 KB (2,680 words) - 13:59, 29 July 2015
• 20.109(S14):Complete Western and prepare damaged DNA (Day4)
  ...ing costs $6-8 per reaction, which can add up if you need to examine, say, 10 or more candidates. Agarose gel electrophoresis, by comparison, costs perha #Add enough TBS-T to cover your membranes – between 10-15 mL should work, but you don't need to measure out this volume. Keep in m
  17 KB (2,797 words) - 13:59, 29 July 2015
• 20.109(S14):DNA repair assays(Day6)
  | 10-21 ...e class, please prepare six 15 mL conicals, labeled 0, 0.1, 0.5, 1, 5, and 10 μM, and six similarly labeled eppendorf tubes.
  17 KB (2,912 words) - 13:59, 29 July 2015
• 20.109(S14):Testing cell viability (Day3)
  ...both live and dead cells; it emits fluorescence in the green channel. SYTO 10 has lower affinity for nucleic acids than does ethidium, and thus is exclud ...orescent dyes such as the fluorescein derivative CFDA-SE are used: about 6-10 divisions can be seen by flow cytometry (see figure at right).
  15 KB (2,507 words) - 13:59, 29 July 2015
• 20.309 Main Page
  |style="width: 10%;"|'''Important dates''' |style="width: 15%;"|'''Assignment due'''
  8 KB (887 words) - 20:47, 16 October 2020
• 20.109(F15):Bacterial amplification of DNA (Day3)
  ... of DNA, but even with highly competent cells only 1 DNA molecule in about 10,000 is successfully transformed. Moreover, the process is somewhat less eff #In an eppendorf tube, combine 10 μL of of your ''DpnI''-digested product with 2.5 μL of loading dye. '
  13 KB (2,207 words) - 11:40, 4 September 2015
• 20.109(F15):Clean and cut DNA (Day2)
  ...the membrane. '''EB''' is one solution we do know everything about. It’s 10 mM Tris, pH 8.5. Since the DNA will solubilize at low salt and high pH, it ... this assignment within ApE. Other program features may help you solve the assignment, but be sure not to "black box" any steps that you aren't sure you also und
  11 KB (1,805 words) - 19:52, 18 September 2015
• 20.109(F15):Journal Club II (Day8)
  ...d a mini-presentation that was focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-rev '''''You will complete this assignment individually.'''''
  12 KB (1,779 words) - 04:42, 3 November 2015
• 20.109(S16):Journal Club II (Day8)
  ...d a mini-presentation that was focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-rev '''''You will complete this assignment individually.'''''
  10 KB (1,596 words) - 18:50, 2 April 2016
• 20.109(S16):Cell preparation for DNA repair assays (Day5)
  ...hematic above. To account for pipetting error, you will add an additional 10% of each component to the mixture. ...hematic above. To account for pipetting error, you will add an additional 10% of each component to the mixture.
  16 KB (2,542 words) - 18:34, 28 March 2016
• 20.109(F16):Clean and cut DNA (Day2)
  ...the membrane. '''EB''' is one solution we do know everything about. It’s 10 mM Tris, pH 8.5. Since the DNA will solubilize at low salt and high pH, it ... this assignment within ApE. Other program features may help you solve the assignment, but be sure not to "black box" any steps that you aren't sure you also und
  11 KB (1,805 words) - 15:11, 18 May 2016
• 20.109(F16):Journal club II (Day 6)
  '''You will complete this assignment individually.''' Please review the 20.109 [http://engineerbiology.org/wiki/ ...d a mini-presentation that was focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-rev
  10 KB (1,515 words) - 16:52, 2 November 2016
• 20.109(F16):Data analysis (Day7)
  .... Note: This will be counted in your homework grade and is due tonight at 10 pm (Thursday, October 6 or Friday, October 7, according to your laboratory ... data slide.''' Use this as a guide to complete the M1D7 in-class homework assignment and for the data slides that within the M1 Data Summary.]]
  5 KB (901 words) - 16:39, 6 October 2016
• 20.109(S17):Journal Club II (Day8)
  '''You will complete this assignment individually.''' Please review the 20.109 [http://engineerbiology.org/wiki/ ...d a mini-presentation that was focused on your research project. For this assignment, you will present work completed by other scientists that has been peer-rev
  12 KB (1,818 words) - 20:50, 6 April 2017
• Assignment 3, Part 1: visualizing actin with fluorescence contrast
  This is Part 1 of [[Assignment 3 Overview| Assignment 3]]. # Add 1 mL of pre-warmed 10 μM CytoD solution and incubate at 37 °C for 15 minutes. Watch the time
  15 KB (2,365 words) - 20:25, 25 February 2020
• Assignment 2: fluorescence microscopy
  [[Category:20.309 assignment 1]] This assignment has 3 parts.
  3 KB (531 words) - 18:23, 14 February 2020
• Assignment 7 Overview
  Short strands of DNA from about 10-50 base pairs in length are called "oligonucleotides" or just "oligos." In ==How to do this lab (assignments 7 through 10)==
  13 KB (1,864 words) - 21:49, 3 April 2018